A total of 740 cycles of intracytoplasmic sperm injection (ICSI) were performed: 625 cycles when < 6 x 10(5) total motile spermatozoa were harvestable from the ejaculate and 115 cycles in cases with a history of previous fertilization failure after classic in-vitro fertilization or subzonal sperm injection. An average of two pronuclei were observed in 63% of the injected oocytes, allowing 725 t...

Four distinct studies were carried out using two data sets of percutaneous epididymal sperm aspiration (PESA) and intracytoplasmic sperm injection (ICSI) procedures performed from March 1993 to January 1997. In study A, an analysis of 181 ICSI treatment cycles following PESA revealed a successful epididymal sperm retrieval rate of 83%. It confirmed that PESA is an effective sperm retrieval meth...

ObjectiveTo identify the risk of stillbirth from in vitro types assisted reproductive technologies compared with spontaneous conception (SC), limited to singleton births.DesignSystematic literature search and chaining on online databases: PubMed, Embase, Scopus.SettingNot applicable.Patient(s)Singleton pregnancies fertilization (IVF) or by IVF intracytoplasmic sperm injection (IVF-ICSI).Interve...

The aim of this prospective study was to evaluate whether couples with total fertilization failure in a previous in-vitro fertilization (IVF) attempt should be offered an additional IVF treatment with elevated insemination concentration or should be treated with intracytoplasmic sperm injection (ICSI). In 23 cycles 228 sibling metaphase II (MII) oocytes were randomly divided: 143 and 85 oocytes...

In these last twenty years, intracytoplasmic sperm injection (ICSI) has efficiently permitted the treatment of male factor infertility (Van Steirteghem et al., 1993); the direct injection of spermatozoa into ooplasm has allowed the embryologist to overcome low sperm motility, poor sperm-Zona Pellucida (ZP) binding, and defective acrosome reaction. Although ICSI has been successfully applied wor...

BACKGROUND There is considerable uncertainty as to the significance of a high sperm DNA fragmentation index (DFI) for achieving a successful pregnancy. METHODS The sperm DFI of 124 patients undergoing 192 IVF cycles and of 96 patients undergoing 155 ICSI cycles was determined using the sperm chromatin structure assay on neat sperm. RESULTS The rate of continuing pregnancies in ICSI cycles (...

OBJECTIVES The aim of the present study was to compare two procedures for sperm selection in ICSI cycles - conventional morphology sperm selection (ICSI-PVP) and chemical selection through Hyaluronan-treated petri dishes (PICSI), when male factor was associated. METHODS The evaluated parameters were semen quality, fertilization and cleavage rates, chemical and clinical pregnancy rates, as wel...

OBJECTIVE The goal of this study was to evaluate the pregnancy potential of immature (metaphase I or germinal vesicle stage) oocytes retrieved in intracytoplasmic sperm injection (ICSI) cycles. METHODS A total of 1,871 couples with infertility underwent 2,984 ICSI cycles. Cycles in which three or fewer oocytes were retrieved were included in this study in order to evaluate the pregnancy poten...

BACKGROUND & OBJECTIVE The objective of the present study was to compare the levels of sperm centrin a centrosomal protein that influences cell migration, in normal fertile donors and in oligoasthenozoospermic males (count 5 million/ml and motility <40%, grade c+d) undergoing intracytoplasmic sperm injection (ICSI) and to correlate with the outcome of ICSI. METHODS The prospective study carri...

BACKGROUND Sperm quality may degrade during transit through the male reproductive tract in some individuals. In this setting surgically retrieved testicular sperm may outperform ejaculated samples for use with in vitro fertilization (IVF) and intracytoplasmic sperm injection (IVF-ICSI). We sought to describe one center's experience with the use of fresh testicular sperm after prior failed IVF-I...