Fertilization and reproduction are central to the survival and propagation of a species. Couples who cannot reproduce naturally have to undergo in vitro clinical procedures. An integral part of these clinical procedures includes isolation of healthy sperm from raw semen. Existing sperm sorting methods are not efficient and isolate sperm having high DNA fragmentation and reactive oxygen species ...

BACKGROUND Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoosperm...

Treatment of demembranated sea-urchin sperm for 1-2 min with 10 microM-N-ethylmaleimide (Mal-NEt) at pH 8.0 prior to reactivation with 1 mM-ATP causes the asymmetry of the flagellar waveform to become desensitized to the presence or absence of Ca2+ in the reactivating medium. In such sperm, changes in concentration of free Ca2+ between 10(-7) M and 10(-3) M have no effect on the asymmetry of th...

Rainbow trout homogametic males, (XX or YY sex genotype), can be obtained, respectively, through masculinisation of genetic females or induced androgenesis. Aim of this study was to compare reproductive potential of neo-males (XX) and super-males (YY) with heterogametic males (XY). We measured spermatozoa motility parameters, sperm concentration, osmolality and characterized protein profiles in...

background: as we all know, sperm has the capacity to take up foreign dna, therefore, sperm mediated gen transfer can be an inexpensive and simple method in animal transgenesis in various species. however, there is not sufficient evidence of dna uptake by ovine spermatozoa. objectives: the purpose of the present study was to examine the uptake of human lysozyme gene contained plasmid (pegfp-ire...

Background: Formaldehyde (FA) is a member of aldehyde family with a simplest organic molecule, used in various industries. Since there is simultaneous exposure of formaldehyde and noise for workers in most of the workplaces and that noise can reinforce the harmful effects of some chemical pollutants. This study aimed to investigate the effects of simultaneous formaldehyde and noise exposure on ...

To evaluate the clinical outcomes of loupe-assisted intussusception vasoepididymostomy (VE) in the treatment of epididymal obstructive azoospermia (EOA), we retrospectively analyzed data from 49 patients with EOA who underwent two-suture longitudinal intussusception vasoepididymostomy (LIVE) between 2000 and 2007. The data included the surgical method, postoperative motile sperm count per ejacu...

The incidence of T. vaginalis infection has been increasing around the world over the past decade. This has lead to insight of the importance of this microorganism as a serious problem in reproductive health outcomes including pelvic inflammatory disease, pregnancy complications and an increased risk of HIV acquisition. For natural fertility, not only optimal semen sample that contains a normal...

OBJECTIVE The purpose of this study was to determine the impact of post-wash total progressive motile sperm count (TPMSC) and semen volume on pregnancy outcomes in intrauterine insemination (IUI) cycles. MATERIAL AND METHODS The retrospective study included a total of 156 cycles (141 couples) and was performed in our center over a 24-month period. The semen parameters were recorded for each m...