The development of monoclonal antibodies (mAbs) for the treatment of disease goes back to the vision of Paul Ehrlich in the late 19th century; however, the first successful treatment with a mAb was not until 1982, in a lymphoma patient. In multiple myeloma, mAbs are a very recent and exciting addition to the therapeutic armamentarium. The incorporation of mAbs into current treatment strategies ...

In an ongoing quest to improve the therapeutic arsenal against cancer, a fourth weapon Intas Biotechnology/Oncology, Plot other than surgery, chemotherapy and radiotherapy has emerged, i.e. targeted therapy. No. 423/P/A/GIDC, Sarkhej Bavla Targeted therapy includes, tyrosine kinase receptor inhibitors (small molecule inhibitors Highway, Moraiya, Tal: Sanand, Ahmedabad-382 210. India like imatin...

INTRODUCTION Characteristics of Monoclonal Antibodies, 203 Production of Monoclonal Antibodies, 207 Developing the Screening Method, 207 Generating Hybridomas, 216 Preparation for Fusions, 217 Fusions, 220 Plating Strategies, 222 Feeding Hybridomas, 224 Supernatant Collection Strategies for Screening, 224 Screening, 225 Expanding and Freezing Positive Clones, 225 Dealing with Contamination, 229...

The 3 murine monoclonal antibodies, Yps1, Yps2 and Yps3 reactive to Y. pseudotuberculosis can be stabilized and all were found to be of IgG type. Monoclonal antibody, Yps1, recognized a glycoprotein antigen of the organism with reactivity at the 55-75 kDa region, while Yps2 and Yps3 recognized protein antigens of Y. pseudotuberculosis 65 kDa and 26-28 kDa molecular weight regions, respectively....

Phosphotyrosine coupled to KLH, BSA, and OVA was used for the production and screening of antibodies to phosphotyrosine. 800 hybridomas secreting antibodies that bound to phosphotyrosine were detected by ELISA. The most reactive 100 of these 800 were tested subsequently for their ability to bind phosphotyrosine-containing proteins on Western blots. Eight stable hybridoma cell lines were selecte...

Monoclonal antibodies (MCAs) can be used to differentiate between normal and neoplastic cells and thus exploited for diagnostic and, ultimately, therapeutic gain. The evidence for the existence of human tumour antigens is reviewed. Several areas of diagnosis are already benefiting from the application of the monoclonal technology. Immunohistology can help the pathologist with difficult diagnost...

Monoclonal antibodies to the capsular polysaccharide of Cryptococcus neoformans produce distinct capsular reactions and have biological activities that are determined by serotype specificity. In the present study, polyclonal rabbit anticapsular antibodies were cross-absorbed to produce serotype specificities similar to those of monoclonal antibodies. The results showed that polyclonal and monoc...

Aims: To measure the content of immunoreactive human pancreatic secretory trypsin inhibitor (irPSTI) in colonic carcinoma and adjacent normal colonic mucosa. Methods: From a stable hybridoma cell line producing monoclonal antibodies specific for human PSTI, a specific enzyme linked immunosorbent assay (ELISA) for human PSTI was developed. In a precipitation assay system these antibodies bound h...

Mucins are of major interest in cell biology, not only are they highly over-expressed in many adenocarcinomas (up to 40-fold increase), but also have important physiological function, and probably more to be determined (1-3). There is much information available on mucins - doubtless because of their unusual structure being heavily glycosylated, but also containing a repeat region rich in the am...

content in L2 lung cells and WI-38 fibroblasts cultivated under hypoxic and normoxic conditions. After 96 h of hypoxic cultivation, PyKi activity was significantly increased in both cell types (L2: normoxia [Po2 = 142 torr], 0.11+0.01 [SD]; hypoxia [Po2 = 14 torr], 0.25 ±0.04 U/,ug DNA, P < 0.01). PyKi content increased proportionately in both cell lines (L2: normoxia, 0.44 +0. 13; hypoxia, 0.9...