abstract background and aims: the human papilloma virus was introduced as the major etiological agent in outbreak of cervical cancer in 1970. since it is very difficult to recognize these viruses and their types using the serological tests and cell culture, molecular methods such as pcr are of great importance. therefore, in this study, our goal was to use a multiple specific pcr assay on l1 an...

objective: in-time diagnosis of streptococcus pneumoniae (s. pneumonia) can play a significant role in decreasing morbidity and mortality rate. applying molecular methods has gained popularity due to the existing limits of routine diagnostic methods. examining the expression of different genes of this bacterium through different molecular methods suggests that lyta gene has a higher sensitivity...

objectives: acinetobacter baumannii is a gram-negative, non-motile aerobic bacterium known as a nosocomial pathogen resisting often to broad range of antibiotics. the pathogen is a serious agent of mortality and morbidity in hospital particularly among immunocompromise patients. treatment and control of its infections is complicated owing to its high antibiotic resistance, survival in various e...

the genus entamoeba contains many species, six of which (entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, entamoeba polecki, entamoeba coli, and entamoeba hartmanni) reside in the human intestinal lumen. entamoeba histolytica is the causative agent of amebiasis in humans with a worldwide distribution. correct detection of e.histolytica is frequently a major concern in diagnostic ...

conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...

Objective(s):To determine the fetal discernment in suspected cases of sex linked recessive disease in the first trimester of pregnancy. Materials and Methods: After collection of 100 Chorionic Villi samples, the DNAs were extracted and baby gender was determined. Meanwhile, after increasing the sensitivity, the system was able to detect the sex of each cell which was obtained by biopsy. Resul...

metabolome analysis is used to evaluate the characteristics and interactions of low molecular weight metabolites under a specific set of conditions. in cirrhosis, hepatocellular carcinoma, non-alcoholic fatty liver disease (nafld) and non-alcoholic steatotic hepatitis (nash) the  liver  does not function thoroughly due to long-term damage. unfortunately the early detection of cirrhosis, hcc, na...

We describe the application of a recently published general event detection framework, called EVE to the challenging task of molecular event detection, that is, the automatic detection of structural changes of a molecule over time. Different types of molecular events can be of interest which have, in the past, been addressed by specialized methods. The framework used here allows different types...