Quantitative effects of altering oxidative phosphorylation and respiration on the activity of the enzyme menadione reductase (NAD[P]H,:2-methyl-i,4-naphthoquinone oxidoreductase, E.C. 1.6.5.2), in stabilized polymorphonuclear neutrophil leucocytes of Amphiuma tridactylum, were studied by amplitude-contrast microscopy and microspectrophotometry. In a general way, the rate of enzymic activity was...

The conjugation of ubiquitin to proteins plays an important role in the regulation of numerous cellular processes. Deregulation of this pathway has been associated with different human disorders including cancer and neurodege nerative diseases. For ubiquitylation, ubiquitin is initially activated by a ubiquitin activating enzyme (E1) at the expense of adenosine triphosphate (ATP; see Figure 1a)...

Previously, we showed that protein kinase B (Akt) activation increases intracellular ATP levels and decreases necrosis in renal proximal tubular cells (RPTC) injured by the nephrotoxicant S-(1, 2-dichlorovinyl)-l-cysteine (DCVC) (Shaik ZP, Fifer EK, Nowak G. Am J Physiol Renal Physiol 292: F292-F303, 2007). This study examined the role of Akt in improving mitochondrial function in DCVC-injured ...

The interest of scientists for the transcription of the eukaryotic genome started four decades ago with the purification of the three RNA polymerase (Pol) enzymes. Pol I, II and III transcribe different species of RNA with the help of several general/basal transcription factors (TFs). Messenger RNA (mRNA) transcription by Pol II has been intensely studied owing first to the protein-coding funct...

Ga(III), In(III) and Tl(III) ions in the presence of different sulfate salts have been successfully separated using 1-(3,4-dihydroxybenzaldehyde)-2-acetylpyridiniumchloride hydrazone (DAPCH) loaded on Duolite C20 in batch and column modes. The obtained modified resin as well as the metal complexes was characterized by elemental analysis and infrared spectra. The extraction isotherms were determ...

Methylglyoxal (MGO), a potent glycating agent, forms advanced glycation end products (AGEs) with proteins. Several diabetic complications including cataract are thought to be the result of accumulation of these protein-AGEs. alpha-Crystallin, molecular chaperone of the eye lens, plays an important role in maintaining the transparency of the lens by preventing the aggregation/inactivation of sev...

Introduction To integrate and execute a proper preventive plan reduce the risk of non-communicable diseases (NCDs), policy makers need to have access both reliable data unique definition metabolic syndrome (MetS). This study was conducted on collected by cross-sectional studies WHO’s STEPwise approach surveillance NCD factors (STEPs) estimate national sub-national prevalence rates MetS in Iran ...

The third National Cholesterol Education Program Adult Treatment Panel (ATP III) defines the metabolic (or insulin resistance) syndrome as the presence in an individual of at least three of the following five risk factors: central or abdominal obesity, hypertriglyceridemia, hypertension, low HDL cholesterol, and high fasting glucose levels. The metabolic syndrome is a major risk factor for card...

OBJECTIVE To examine the association of the metabolic syndrome, defined by World Health Organization (WHO) and Adult Treatment Panel III (ATP-III) criteria, and its components with coronary artery calcium (CAC) progression. RESEARCH DESIGN AND METHODS Participants were 338 older community-dwelling men and women without known heart disease who had measurements of heart disease risk factors and...