More than 80% of the milk stored in the udder, i.e. the alveolar fraction, only available after milk ejection is induced by the release of oxytocin and myoepithelial contraction. Milk ejection is induced by tactile teat stimulation, either manually or by the milking machine. The time from the start of a tactile stimulation until the occurrence of milk ejection spans from 40 s up to more than 2 ...

e objective of the study was to determine psychrotrophic bacteria counts (PBC) and total bacterial counts (TBC) in bulk milk samples collected during a longer period (1999 to 2000). Two sets of samples were analysed. Set 1, including samples collected in three herds, was analysed by the Central Laboratory at České Budějovice. TBC was determined using the apparatus Bactoscan, and PBC by culture...

Eight Holstein cows were used to determine the relationship among milk urea nitrogen (MUN), plasma urea nitrogen (PUN), and feeding time. We first established that MUN concentrations were similar in concentration among quarters by comparing milk samples from each quarter just before milking. In order to determine if collecting a sample of milk from a quarter influenced the MUN in samples taken ...

In the dairy industry world wide, several hundred types of liners are used to milk dairy cows. The enormous amount of liner types with different characteristics in size, form, consistency and other design and manufacturing characteristics indicate that no ‘best’ liner has been identified in the last 60 or so years since machine milking has become the common method for milk harvesting. Mechanica...

Milking dairy cows four times daily (4×) instead of twice daily (2×) during early lactation stimulates an increase in milk yield that partly persists through late lactation; however, the mechanisms behind this response are unknown. We hypothesized that the acute mammary response to regular milkings would be transient and would involve different genes from those that may be specifically regulate...

The population of farms using automatic milking systems (AMS) has grown from the first installation a dairy farm in the Netherlands in 1992 to more than 8000 milking units on more than 2400 farms today The vast majority of these farms are in north-western Europe, with the Netherlands having the largest installed base and Scandinavia showing the fastest growth rate in the past few years (Lind, 2...

: Friesian-type dairy cows were milked with different machine settings to determine the effect of these settings on teat tissue reaction and on milking characteristics. Three teat-cup liner designs were used with varying upper barrel dimensions (wide-bore WB = 31.6 mm; narrow-bore NB = 21.0 mm; narrow-bore NB1 = 25.0 mm). These liners were tested with alternate and simultaneous pulsation patter...

The objective of this study was to assess the presence of a Listeria monocytogenes-containing biofilm in milking equipment as a potential source of bulk tank milk contamination on a dairy farm where milk contamination had been previously documented. Samples were collected from milking equipment and milking parlor premises on 4 occasions and analyzed for the presence of L. monocytogenes. Pulsed-...

The use of PCR tests as diagnostics for intramammary infections (IMI) based on composite milk samples collected in a non-sterile manner at milk recordings is increasing. Carryover of sample material between cows and non-aseptic PCR sampling may be incriminated for misclassification of IMI with Streptococcus agalactiae (S. agalactiae) in dairy herds with conventional milking parlours. Misclassif...