Two randomized controlled trials published in 2002 demonstrated that mild hypothermia induced after resuscitation from cardiac arrest decreased mortality and improved neurologic outcomes. Although therapeutic hypothermia is relatively easy to implement, side effects and special nursing considerations are associated with this therapy. This article discusses the process of developing and implemen...

OBJECTIVE Glutathione (GSH) has a central role in the defence against oxidative damage. This study was carried out to investigate any change in erythrocyte GSH levels in a population of patients with acute myocardial infarction (AMI) and compare them with levels in normal healthy subjects. METHOD GSH levels were determined in erythrocytes of one hundred and seventy six patients with AMI (age:...

Glutathione (γ-glutamylcysteinyl-glycine, GSH) has vital functions as thiol redox buffer and cofactor of antioxidant and detoxification enzymes. Plasmodium falciparum possesses a functional GSH biosynthesis pathway and contains mM concentrations of the tripeptide. It was impossible to delete in P. falciparum the genes encoding γ-glutamylcysteine synthetase (γGCS) or glutathione synthetase (GS),...

the clinical uses of gentamicin have so far been restricted due to nephrotoxicity and ototoxicity. the exact mechanism of nephrotoxicity is still unknown; however, it appears that free radicals may be involved. methionine has previously been shown to alleviate oxidative stress involved in ototoxicity due to its antioxidant properties. therefore, the effect of methionine supplementation on the g...

Background To study the nursing method of mild hypothermia therapy for moderate or severe hypoxic-ischemic encephalopathy (HIE) in neonates. Methods According to the inclusion and exclusion criteria, 48 patients were selected from Nanfang Hospital from December 2015 to October 2016 and randomly divided into the control group (n=24) and observation group (n=24). The control group received rout...

The modulation of the redox microenvironment is an important regulator of immune cell activation and proliferation. To investigate immune cell redox status in autism we quantified the intracellular glutathione redox couple (GSH/GSSG) in resting peripheral blood mononuclear cells (PBMCs), activated monocytes and CD4 T cells and the extracellular cysteine/cystine redox couple in the plasma from 4...

abstract background: in 1958, silverman demonstrated that maintenance ofbody temperature reduces mortality in low birth weight infants. from the early 1990s it was already recognized that adequate environmental warmth was essential in the case of newborns. however, neonatal hypothermia continues to be a significant issue in developing countries. methods: in order to describe the incidence and s...

An enzymatic recycling method has been applied to the measurement of total and oxidized glutathione with a centrifugal analyzer. When the reduced form of glutathione (GSH) was masked with 2-vinylpyridine to measure the oxidized glutathione (GSSG), the time to ensure full derivatization was three times longer than has been reported. The method is quick, simple, accurate, and precise (1.27% for G...