The microflora of Tibetan kefir grains was investigated by culture- independent methods. Denaturing gradient gel electrophoresis (DGGE) of partially amplified 16S rRNA for bacteria and 26S rRNA for yeasts, followed by sequencing of the most intense bands, showed that the dominant microorganisms were Pseudomonas sp., Leuconostoc mesenteroides, Lactobacillus helveticus, Lactobacillus kefiranofaci...

six rumen fistulated adult sheep were used to assess the effect of tannins (hydrolysable tannin; hts) in oak leaves (quercus libani oliv.) on ruminal fermentation parameters in a change-over design experiment for 28 days in 3 periods. polyethylene glycol (peg) was used to deactivate the tannins. the three dietary treatments were control (alfalfa hay, barley grain, wheat bran, wheat straw); ol (...

Most of the information on the gastrointestinal microflora of antarctic birds was obtained during the great decade of South-Polar exploration following the turn of the century. The report of Levin (1899) that bacteria could not be found in the intestines of arctic birds at Spitzbergen prompted similar studies by the naturalists and ship's physicians on the Antarctic expeditions. Ekel6f (1908) o...

We have developed an inexpensive, standardized paper chromogenic array (PCA) integrated with a machine learning approach to accurately identify single pathogens (Listeria monocytogenes, Salmonella Enteritidis, or Escherichia coli O157:H7) multiple (either in monocultures, cocktail culture), the presence of background microflora on food. Cantaloupe, commodity significant volatile organic compoun...

We have previously conducted a microflora analysis and examined the biofilm-forming activity of bacteria isolated from toilet bowl biofilms. In the present investigation, to reveal the strain involved in the formation of black dirt in toilet bowls, we performed a microflora analysis of the bacteria and fungi isolated from the black dirt of toilet bowls at ten homes. Among samples from different...

Capsaicin (CAP) and Yucca schidigera extract (YSE) are two types of plant extracts that can change rumen fermentation. This study was conducted to investigate whether supplementation beef cattle diets with CAP YSE for 90 days would affect fermentation microflora. Forty-five healthy Angus steers (initial body weight = 510.54 ± 41.27 kg) were divided into three groups: control (CON), CAP, YSE. Am...

Effective antimicrobial stewardship requires a better understanding of the impact different antibiotics on gut microflora. Studies with humans are confounded by large interindividual variability and difficulty in identifying control cohorts.