lactoferrin (lf) is an iron-binding multi-functional glycoprotein which has numerous physiological functions such as iron transportation, anti-microbial activity and immune response. in this study, different in silico approaches were exploited to investigate lf protein properties in a number of mammalian species. results showed that the iron-binding site, dna and rna-binding sites, signal pepti...

Introduction The in situ method is used widely to determine ruminal degradability of feed CP and DM. It is an attractive alternative to in vivo methods because it is simple, inexpensive, and rapid. However, several factors influence in situ results, the most important of which is microbial contamination of feed residues after incubation in the rumen. Degradation of forage CP and DM is systemati...

IMPORTANCE Bevacizumab acquired from compounding pharmacies for intravitreal injection may cause infectious and noninfectious inflammation. In addition to safety issues, the drug itself may have variable efficacy associated with product aliquoting, handling, and distribution. OBJECTIVE To conduct surveillance cultures, evaluate endotoxin levels, and assess protein concentrations of bevacizuma...

Protein synthesis by rumen microbes plays an important role in ruminant nutrition. Microbial proteins that are not recycled flow out of the rumen and account for more than 50% of the total proteins entering the intestines. Determination of microbial nitrogen in digesta is based on the isolation of a representative sample of rumen microbes and on the use of specific markers (purine bases, RNA, 1...

Project Goals (Abstracts 143-145): The aim of this project is to develop integrated genomic and proteomic (proteogenomic) methods to study virus-host dynamics in bioreactor-grown and natural microbial biofilm communities. We will utilize simultaneous metagenomic analysis of CRISPRs (the adaptive microbial immune system) and viruses to examine the co-evolution and dynamics of viruses and their m...

The rumen is an open fermentation chamber, inhabited by microorganisms that anaerobically digest complex components of feedstuffs and generate fermentation products (mainly acids) and microbial cell mass for utilization by the host. The absorption of fermentation products, mainly VFA, from the rumen into the blood, and outflow of microbial cells to the abomasum and small intestine provide the a...

Phytic acid is an antinutritional compound that chelates P and essential divalent cations such as Fe, Ca, Zn in cereals major staple crops wheat, maize, rice, sorghum. As a result, these cannot be absorbed by monogastric animals or humans: phytic has inhibitory effect on nutrient uptake its levels are negatively correlated with protein starch digestibility. However, can degraded the action of e...

In dairy industry there has always been great concern about the microbial and protein quality of liquid milk fractions obtained by microfiltration (MF). The objectives research were to study impact MF on evolution microfiltered (MFM), micellar casein concentrates (LMCC) serum permeates (SP) during storage at 4 °C. This was carried out with three different batches using diafiltration. Samples ch...

We previously showed that the chimeric proteins of microbial rhodopsins, such as light-driven proton pump bacteriorhodopsin (BR) and Gloeobacter rhodopsin (GR) that contain cytoplasmic loops of bovine rhodopsin, are able to activate Gt protein upon light absorption. These facts suggest similar protein structural changes in both the light-driven proton pump and animal rhodopsin. Here we report t...