PURPOSE A combination therapy for combined injury (CI) using a non-specific immunomodulator, synthetic trehalose dicorynomycolate and monophosphoryl lipid A (STDCM-MPL), was evaluated to augment oral antimicrobial agents, levofloxacin (LVX) and amoxicillin (AMX), to eliminate endogenous sepsis and modulate cytokine production. MATERIALS AND METHODS Female B6D2F(1)/J mice received 9.75 Gy coba...

Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) have been implicated as key mediators in inflammation, morbidity, and mortality associated with sepsis. We examined the role of IL-6 and TNF-α signaling on hypothermia, fever, cachexia, anorexia, and survival during sepsis induced by cecal ligation and puncture (CLP) in male and female gene knockout mice. Male wild-type mice developed an ...

Generalist mice are key species for the long-term dynamics of fragmented forests due to their dual role as seed dispersers or predators dominant trees. Wood mice, Apodemus sylvaticus, usually act a net predator in woodlots higher winter densities and earlier reproduction than forests. Here we analyze recruitment expectations young born relation food availability through an index developmental s...

The strain-dependent survival of cystic fibrosis (CF) knockout mice has been used to map a modifier of CF, Cfm1, in mice and, subsequently, in humans. To identify additional modifiers of the CF phenotype, in this study, the survival of F2 CF mice derived from a cross between congenic C57BL/6J CF and BALB/cJ CF heterozygotes was followed up to 12 wk of age. A genome-wide linkage scan completed i...

OBJECTIVES To assess the effects of corticosteroid administration on functional recovery and cell survival in the facial motor nucleus (FMN) following crush injury in adult and juvenile mice and to evaluate the relationship between functional recovery and facial motoneuron survival. METHODS A prospective blinded analysis of functional recovery and cell survival in the FMN after crush injury i...

BACKGROUND Cyclic acyldepsipeptides (ADEPs) are a novel class of antibacterial agents, some of which (e.g., ADEP 4) are highly active against Gram-positive bacteria. The focus of these in vivo studies is ADEP B315, a rationally designed compound that has the most potent in vitro activity of any ADEP analog reported to date. METHODS In vivo efficacy experiments were performed using lethal intr...

background: tumor associated antigens can be viably used to enhance host immune response.objectives: the immunomodulatory effect of biogenic selenium nanoparticles (senps) was compared between treated and untreated mice with crude antigens of 4t1 cells.materials and methods: female inbred balb/c mice (60) were injected by cancinogenic 4t1 cells causing breast cancer. after 10 days, all tumor be...

background: severe and fatal complications of toxoplasmosis urge development of effective vaccines against the disease. the current study was performed to evalu­ate cocktail dna vaccine containing plasmids encoding gra5, sag1, and rop2 genes of toxoplasma gondii in balb/c mice in tarbiat modares university in 2012. methods: the plasmids containing complete gra5, sag1, and rop2 genes were mass e...

developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. to accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and fsh concentrations. six-week-old preantral follicles (95 ± 5 µm) were cultured...

Purpose Induced pluripotent stem cells (iPSCs) are promising candidates for regenerative medicine. To overcome the allogeneic immune barrier, we created hypo-immune iPSCs using CRISPR/Cas9 editing strategies and demonstrate long-term survival of allografts without need immunosuppression. Methods Hypoimmunogenic mouse human were generated via technology by knocking out major histocompatibility c...