A molybdenum reducing bacterium with the novel ability to decolorise the azo dye Metanil Yellow is reported. Optimal conditions for molybdenum reduction were pH 6.3 and at 34°C. Glucose was the best electron donor. Another requirement includes a narrow phosphate concentration between 2.5 and 7.5 mM. A time profile of Mo-blue production shows a lag period of approximately 12 hours, a maximum amo...

OBJECTIVES The use of colistin in the treatment of life-threatening Gram-negative infections is associated with a high rate of nephrotoxicity that is dose limiting. This study aimed to examine the nephroprotective effect of ascorbic acid against colistin-induced nephrotoxicity. METHODS Rats were treated intravenously twice daily with saline, colistin (cumulative dose of 36.5 mg/kg), a combina...

A near-infrared fluorescent probe based on methylene blue (p-NBMB) was developed for the detection of nitroreductase. Conjugating methylene blue with a p-nitrobenzyl moiety enables it to be activated by nitroreductase-catalyzed 1,6-elimination, resulting in the release of an active methylene blue fluorophore.

The aim of this study was to investigate the effect of storage at -4 0 C on the ascorbic acid (vitamin C) content of grapes (Vitis vinifera) for 1, 2, 5 and 8 months. A sensitive UV-VIS spectrometric method based on Prussian Blue reaction was applied for vitamin C determination and some kinetic calculations were presented. The varieties of red and white grapes used were from Murfatlar vineyard:...

INTRODUCTION Methylene blue dye has been used worldwide successfully with few complications in breast surgery. We present two different complications involving methylene blue: 1) skin and parenchymal necrosis when dye was injected in a subdermal fashion and 2) Mycoplasma infection caused by contaminated methylene blue in breast reduction surgery. METHODS We present two cases seen at the Unive...

Perinatal asphyxia induces retinal lesions, generating ischemic proliferative retinopathy, which may result in blindness. Previously, we showed that the nitrergic system was involved in the physiopathology of perinatal asphyxia. Here we analyze the application of methylene blue, a well-known soluble guanylate cyclase inhibitor, as a therapeutic strategy to prevent retinopathy. Male rats (n = 28...

Oxidation–reduction titrations and potentiometric methods provide a convenient experimental means to quantitatively determine compositions of unknown mixtures containing oxidizable/reducible species. Over the past decade, numerous titration methods have appeared in this Journal (1–6) and in standard laboratory manuals (7–10) involving common titrants such as potassium dichromate, iodine, potass...

A glucose sensor was developed by electrocopolymerization using pyrroles containing a tris-bipyridine (bpy) osmium complex (Os-py), pyrrole (py), pyrrole propanoic acid (PPA) and glucose oxidase (GOx) to improve the key performance characteristics, such as the sensitivity, selectivity, and long-term stability. Tris-bipyridine osmium pyrrole complexes with four different methylene moieties were ...

Various calorimetric methods of estimating epinephrine have been described by different workers using different chemical reagents such as potassium persulfate (I), mercuric chloride (2-4), potassium iodate (5), ammonium molybdate (6), diazobenzenesulfonic acid (7), and the ninhydrin test (8). None of the methods, however, is satisfactory for the estimation of epinephrine in blood. Some are not ...