1. In kidney-cortex slices from the well-fed rat, glucose (5mm) supplied 25-30% of the respiratory fuel; in the starved state, the corresponding value was 10%. These results are based on measurements of the net uptake of glucose and of the specific radioactivity of labelled carbon dioxide formed in the presence of [U-(14)C]-glucose. 2. Added acetoacetate (5mm) or butyrate (10mm) provided up to ...

I. Lipid peroxides have been measured by a micro-iodimetric method in pancreas, lung, adrenal, leg muscle, spleen, brain, small intestine and uterus of vitamin E-deficient and vitamin E-supplemented rats. There were no differences due to the vitamin E status of the rats. 2. The decay of [14C]~-a-tocopherol in peroxidizing methyl oleate, maize oil methyl esters and cod-liver oil methyl esters wa...

Kedelai adalah produk pangan bernilai gizi tinggi yang banyak dikonsumsi oleh masyarakat Indonesia. Pada penelitian ini, kedelai dimodifikasi dengan cara perkecambahan. Komponen zat akan berubah menjadi senyawa-senyawa lebih sederhana selama proses perkecambahan sehingga mudah dicerna bagi tubuh manusia. Selain itu, juga dapat meningkatkan kapasitas antioksidan. Tujuan dari ini menentukan kondi...

Conventional esterification of fats/waste oils with high free fatty acids is time-consuming and energy intensive. In this study, a novel liquid-phase plasma discharge (LPPD) reactor was designed evaluated for rapid to convert oleic acid biodiesel. Three LPPD operating parameters, i.e., feeding flowrate, methanol molar ratio (M/O), sulfuric weight (A/O), were all at five levels (1.2, 1.8, 2.4, 3...

The peroxisome, sole site of beta-oxidation in Saccharomyces cerevisiae, is known to be required for optimal growth in the presence of fatty acid. Screening of the haploid yeast deletion collection identified approximately 130 genes, 23 encoding peroxisomal proteins, necessary for normal growth on oleic acid. Oleate slightly enhances growth of wild-type yeast and inhibits growth of all strains ...

Saturated fatty acids are implicated in the development of insulin resistance, whereas unsaturated fatty acids may have a protective effect on metabolism. We tested in primary human myotubes if insulin resistance induced by saturated fatty acid palmitate can be ameliorated by concomitant exposure to unsaturated fatty acid oleate. Primary human myotubes were pretreated with palmitate, oleate or ...

AIMS To develop a method to assess the relative binding of fatty acid ethyl esters (FAEE) and free fatty acids (FFA) to albumin, and to determine if binding affinity is related to fatty acid chain length and or degree of saturation. METHODS Radiolabelled ethyl-[14C] oleate bound to albumin was challenged with various ratios of FFA to FAEE. The displacement of ethyl-[14C] oleate by FFA was vis...

Because excess triacylglycerol (TAG) in nonadipose tissues is closely associated with the development of insulin resistance, interest has increased in the metabolism of long-chain acyl-CoAs toward beta-oxidation or the synthesis and storage of TAG. To learn whether a mitochondrial isoform of glycerol-3-phosphate acyltransferase (mtGPAT1) competes with carnitine palmitoyltransferase I (CPT I) fo...

Diabetes mellitus, obesity, and dyslipidemia increase risk for cardiovascular disease, and expose the heart to high plasma fatty acid (FA) levels. Recent studies suggest that distinct FA species are cardiotoxic (e.g., palmitate), while others are cardioprotective (e.g., oleate), although the molecular mechanisms mediating these observations are unclear. The purpose of the present study was to i...

To determine the role of cholinergic reflexes on pancreatic secretory response to food, we studied the effect of atropine on amylase secretion in response to the octapeptide of cholecystokinin (CCK8) and to intraintestinal oleate. Four studies were done in six healthy volunteers. The duodenal content was aspirated by a double lumen tube while synthetic secretin (41 pmol/kg/h) was infused as a b...