نتایج جستجو برای: methyl acetoacetate

تعداد نتایج: 111325  

Journal: :The Journal of biological chemistry 1982
N G Forman D F Wilson

The reaction 3-hydroxybutyrate (3-OH-butyrate) + 2 cytochrome c + ~ + 2 ADP + 2 Pi acetoacetate + 2 cytochrome e+’ + 2 ATP was used to study the energy relationships of electron transfer in the forward and reverse directions in suspensions of isolated rat liver mitochondria. Reduction of cytochrome c was monitored continuously at 550-540 nm while aliquots were taken to measure transmembrane pH ...

Journal: :The Biochemical journal 1990
A Gorchein

A specific assay for 5-aminolaevulinate synthase activity is described, with a sensitivity comparable with that of radiochemical assays. It is based on measurement by g.l.c. with electron-capture detection of the pentafluorobenzyl ester of the ethyl acetoacetate pyrrole derivative of 5-aminolaevulinic acid, and of the corresponding compound from 6-amino-5-oxohexanoic acid used as internal stand...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه شیراز 1354

ترکیب 4-hydroxymethyl-(2,3)-benzotetrahydrooxpin toluenesulfonate 11 یکی از اعضای تاسیلیت نوع اول 7 بصورت زیر سنتز شده است : methyl crotonate 1 را بطریق معمولی از اسیدکروتونیک تهیه کرده و توسط nbs در موقعیت r برمومه شد. جسم حاصل methyl-r-bromocrotonate، 2 با فنل ترکیب شده و methyl-r-phenoxy crotonate، 3 بدست آمد که این جسم توسط هیدروژن در حضور کاتالیزور به methyl-phenoxy butyrate، احیا گردید. ه...

Journal: :Biochemistry and Biophysics Reports 2021

Ahmad Erfanmanesh Hadi Gholampour, Mahdi Jahantigh Peyman Asadian

Backgrounds: Subclinical ketosis can cause greater economic loss due to a lack of clinical symptoms. Objectives: The present study was aimed to design a chemical method for measuring serum ketone bodies in the affected subclinical cows. Methods: Acetoacetate concentrations were measured using a nitroprusside reaction and b-hydroxy butyric acid (BHBA) which was oxidized to acetoacetate using nan...

Journal: :The Journal of biological chemistry 1954
R F WITTER M A COTTONE E STOTZ

The relative importance of oxidation through the Krebs cycle or of conversion to acetoacetate for the oxidation of fatty acids in liver is unknown. Attempts have been made to answer this question by studies of the products of the oxidation of fatty acids in particulate systems (l-7). In most of the experiments this process has required the presence not only of adenine nucleotides, magnesium ion...

Journal: :The Journal of biological chemistry 2011
Michael J MacDonald Melissa J Longacre Scott W Stoker Mindy Kendrick Ansaya Thonpho Laura J Brown Noaman M Hasan Sarawut Jitrapakdee Toshiyuki Fukao Matthew S Hanson Luis A Fernandez Jon Odorico

Anaplerosis, the net synthesis in mitochondria of citric acid cycle intermediates, and cataplerosis, their export to the cytosol, have been shown to be important for insulin secretion in rodent beta cells. However, human islets may be different. We observed that the enzyme activity, protein level, and relative mRNA level of the key anaplerotic enzyme pyruvate carboxylase (PC) were 80-90% lower ...

Journal: :Experimental gerontology 2017
E Croteau C A Castellano M Fortier C Bocti T Fulop N Paquet S C Cunnane

INTRODUCTION Deteriorating brain glucose metabolism precedes the clinical onset of Alzheimer's disease (AD) and appears to contribute to its etiology. Ketone bodies, mainly β-hydroxybutyrate and acetoacetate, are the primary alternative brain fuel to glucose. Some reports suggest that brain ketone metabolism is unchanged in AD but, to our knowledge, no such data are available for MCI. OBJECTI...

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