We have already purified membrane-bound, electron transport-linked, D-glucose dehydrogenase from Pseudomonas jluorescens [I], and found that the enzyme had an unknown prosthetic group differing from NAD(P) and flavin. Furthermore, it appears that alcohol [2], aldehyde [3] and D-glucose [4] dehydrogenases isolated in our laboratory from Gluconobacter suboxydans also have the same prosthetic grou...

Bacillus methanolicus can efficiently utilize methanol as a sole carbon source and has an optimum growth temperature of 50 degrees C. With the exception of mannitol, no sugars have been reported to support rapid growth of this organism, which is classified as a restrictive methylotroph. Here we describe the DNA sequence and characterization of a 19,167-bp circular plasmid, designated pBM19, iso...

A modifier protein (M-protein), which increases the affinity of methanol dehydrogenase (MDH) for alcohols but decreases its affinity for formaldehyde, has been partially purified from Methylophilus methylotrophus and Paracoccus denitrificans. Analysis was complicated by non-protein factors in bacterial extracts that are able to mimic M-protein in one of its functions-that of increasing the acti...