A culture-independent molecular phylogenetic approach was used to survey constituents of microbial communities associated with an aquifer contaminated with hydrocarbons (mainly jet fuel) and chlorinated solvents undergoing intrinsic bioremediation. Samples were obtained from three redox zones: methanogenic, methanogenic-sulfate reducing, and iron or sulfate reducing. Small-subunit rRNA genes we...

The effects of elevated atmospheric CO2 concentration on soil microbial communities have been previously recorded. However, limited information is available regarding the response of methanogenic communities to elevated CO2 in freshwater marshes. Using high-throughput sequencing and real-time quantitative PCR, we compared the abundance and community structure of methanogens in different compart...

introduction: antibiotics have the potential to adversely affect the microbial community. for anaerobic digestion, a sufficient methanogenic population needs to be preserved in the system. the main aim of this study was determination of inhibitory concentration of oxytetracycline on methanogenic bacteria. methods: a 120 ml jacketed bioreactor with a 90 ml working volume was inoculated granular ...

It was recognized only recently that subglacial ecosystems support considerable methanogenic activity, thus significantly contributing the global methane production. However, only limited knowledge is available on the physiological characteristics of this kind of methanogenic community because of the technical constraints associated with sampling and cultivation under corresponding environmenta...

Obtaining a reliable estimation of the methane potential of organic waste streams in anaerobic digestion, for which a biochemical methane potential (BMP) test is often used, is of high importance. Standardization of this BMP test is required to ensure inter-laboratory repeatability and accuracy of the BMP results. Therefore, guidelines were set out; yet, these do not provide sufficient informat...

UNLABELLED Methanothermobacter marburgensis is a strictly anaerobic, thermophilic methanogenic archaeon that uses methanogenesis to convert H2 and CO2 to energy. M. marburgensis is one of the best-studied methanogens, and all genes required for methanogenic metabolism have been identified. Nonetheless, the present study describes a gene (Gene ID 9704440) coding for a putative NAD(P)H quinone ...

Potential rates of aerobic respiration, denitri®cation, sulfate reduction and methanogenesis were investigated in 10 di€erent wetland soils with a wide range of biogeochemical characteristics, with the objective of determining relationships between process rates and soil properties. Electron acceptor amendments to methanogenic soils caused gradual (1±13 d) to immediate transitions in electron ̄...

The effect of adding alum water treatment residues (WTR) on the methanogenic activity in digestion primary domestic wastewater sludge was evaluated through laboratory experiments sedimentation columns, using total suspended solids (TSS) concentrations from 0.37 to 1.23 g/L. addition WTR clarifiers can benefit its effluent quality terms colour, turbidity, chemical oxygen demand (COD), and TSS. H...

The mineralization of organic matter in anoxic environments relies on the cooperative activities of hydrogen producers and consumers obligately linked by interspecies metabolite exchange in syntrophic consortia that may include sulfate reducing species such as Desulfovibrio. To evaluate the metabolic flexibility of syntrophic Desulfovibrio to adapt to naturally fluctuating methanogenic environm...

The methanogenic biodegradation of crude oil involves the conversion of hydrocarbons to methanogenic substrates by syntrophic bacteria and subsequent methane production by methanogens. Assessing the metabolic roles played by various microbial species in syntrophic communities remains a challenge, but such information has important implications for bioremediation and microbial enhanced energy re...