Metabolic profiling of ligands for the chemokine receptor CXCR3 by liquid chromatography–mass spectrometry coupled to bioaffinity assessment. Abstract Chemokine receptors belong to the class of G protein−coupled receptors and are important in the host defense against infections and inflammation. However, aberrant chemokine signaling is linked to different disorders such as cancer, central nervo...

Targeted gene inactivation and metabolic profiling revealed that the cryptic PKS-NRPS gene cluster in the genome of the plant commensal Pseudomonas fluorescens Pf-5 codes for the biosynthesis of antiproliferative and antifungal rhizoxin derivatives.

Due to impressive achievements in genomic research, the number of genome sequences has risen quickly, followed by an increasing number of genes with unknown or hypothetical function. This strongly calls for development of high-throughput methods in the fields of transcriptomics, proteomics and metabolomics. Of these platforms, metabolic profiling has the strongest correlation with the phenotype...

It is important to identify patients with Maturity-onset diabetes of the young (MODY) as a molecular diagnosis determines both treatment and prognosis. Genetic testing is currently expensive and many patients are therefore not assessed and are misclassified as having either type 1 or type 2 diabetes. Biomarkers could facilitate the prioritisation of patients for genetic testing. We hypothesised...

Plants synthesize diverse diterpenoids with numerous functions in organ development and stress resistance. However, the role of glandular trichome (GT) GT-localized biosynthesis plants remains unknown. Here, identification 10 diterpene synthases (diTPSs) revealed diversity diterpenoid Artemisia annua. Protein–protein interactions (PPIs) between AaKSL1 AaCPS2 plastids highlighted their potential...

Background: Drug-induced hepatotoxicity is one of the major reasons for drug recall and hence it is of major concern to the FDA and consumers. Overdose of acetaminophen (APAP) can cause acute hepatic injury. The current clinical biomarkers of liver injury are insufficient in predicting the extent of injury; thus novel biomarkers are needed to integrate with the current biomarkers for better ris...

We present a step towards the metabolome-wide computational inference of cellular metabolic reaction networks from metabolic profiling data, such as mass spectrometry. The reconstruction is based on identification of irreducible statistical interactions among the metabolite activities using the ARACNE reverse-engineering algorithm and on constraining possible metabolic transformations to satisf...

Simvastatin (SV) is a semisynthetic derivative of lovastatin (LV), which biosynthetically produced from the fungus Aspergillus terreus and has high log p value (log = 4.39)and thus hepatic extraction efficacy in controlling cholesterol synthesis. The current study was undertaken to investigate metabolic profile SV using various mass spectrometry (MS) platforms. Metabolic profiling studied vitro...

Objective(s): Alzheimer’s disease (AD) is dysfunction of the central nervous system and as a neurodegenerative disease. The objective of this work is to investigate metabolic profiling in the serum of animal models of AD compared to healthy controls and then to peruse the role of ghrelin as a therapeutic approach for the AD.Materials and Methods: Nuclear magnetic resonance (NMR) technique was u...

Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. ABSTRACT Although considerable progress has been made towards understanding glioblastoma biology through large-scale genetic and protein expression analyses, little is known about the underlyin...