TLS-ERG fusion protein is derived from the t(16;21) translocation found in human myeloid leukemia. Here, we show that retroviral transduction of TLS-ERG confers a growth advantage to L-G myeloid progenitor cells and blocks terminal differentiation. We found that the level of cyclin-dependent kinase 1 (Cdk1) protein was significantly decreased in controls but unchanged in TLS-ERG-expressing cell...

BACKGROUND This study aimed to evaluate the association of recurrent molecular alterations in prostate cancer, such as ERG rearrangements and phosphatase and tensin homolog gene (PTEN) deletions, with oncologic outcomes in patients with prostate cancer treated with brachytherapy. METHODS Ninety-two men underwent I-125 brachytherapy with a 145 Gy delivered dose between 2000 and 2008. Pretreatm...

ERG (Ets-related gene) is an ETS transcription factor that has recently been shown to regulate a number of endothelial cell (EC)-restricted genes including VE-cadherin, von Willebrand factor, endoglin, and intercellular adhesion molecule-2. Our preliminary data demonstrate that unlike other ETS factors, ERG exhibits a highly EC-restricted pattern of expression in cultured primary cells and seve...

BACKGROUND To investigate if microRNAs (miRNAs) play a role in regulating h-ERG trafficking in the setting of chronic oxidative stress as a common deleterious factor for many cardiac disorders. METHODS We treated neonatal rat ventricular myocytes and HEK293 cells with stable expression of h-ERG with H2O2 for 12 h and 48 h. Expression of miR-17-5p seed miRNAs was quantified by real-time RT-PCR...

Solar magnetic tornadoes are known to be one of the mass and energy transport mechanisms from the lower solar atmosphere into the upper layers of the solar corona. A bright spiral structure with two arms is observed using high-cadence EUV images of 171, 193 and 304 Ǻ channels of Atmospheric Imaging Assembly (AIA) aboard the Solar Dynamics Observatory (SDO) on 10th of July 2011 for three hours. ...

Whether vitamin D is chemopreventive and/or has potential therapeutically in prostate cancer is unresolved. One confounding factor is that many prostate cancers express a TMPRSS2:ERG fusion gene whose expression is increased both by androgens and by vitamin D receptor (VDR) activation. Two challenges that limit VDR agonist use clinically are hypercalcemia and the cooperation of VDR with ERG to ...

The interaction of transcription factors with specific DNA sequences is critical for activation of gene expression programs. In endothelial cells (EC), the transcription factor NF-κB is important in the switch from quiescence to activation, and is tightly controlled to avoid excessive inflammation and organ damage. Here we describe a novel mechanism that controls the activation of NF-κB in EC. ...

half of all prostate cancers in the Western countries harbor gene fusions that involve regulatory sequences of the androgen receptor (AR)-responsive genes (predominantly TMPRSS2) and protein coding sequences of nuclear transcription factors of the ETS gene family (predominantly ERG). This leads to unscheduled androgen-dependent expression ofETS-related transcription factors in tumor cell-specif...

Electroretinography (ERG) is a well-established diagnostic procedure for objectively evaluating retinal function. In this study, ERG in beagle dogs, which are a popular experimental animal, was performed to determine the normal range of ERG variables and assess differences between the left and right eyes. ERG findings including rod, combined rod-cone, single-flash cone, and 30-Hz flicker respon...

Purpose: To assess differentially methylated "landscapes" according to prostate cancer Gleason score (GS) andERGoncogene expression status, and to determine the extent of polycomb group (PcG) target gene involvement, we sought to assess the genome-wideDNAmethylation profile of prostate cancer according to Gleason score and ERG expression. Experimental Design:Genomic DNA from 39 prostate cancer ...