Article history: Received 21 June 2016 Accepted 21 June 2016 Available online 23 June 2016 (Akira et al., 2006). Innate immunity relies on the binding of PAMPs to receptors on immune cells and to serum proteins, such as mannosebinding lectin (Akira et al., 2006; Ip et al., 2009). Cartwright et al. developed an ELISA using an engineered immunoglobulin domain fused to domains of mannose-binding l...

Objective(s) Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA)...

Multivalent protein binding plays an important role not only in biological recognition but also in biosensor preparation. Infrared reflection absorption spectroscopy and surface plasmon resonance techniques have been used to investigate concanavalin A (Con A) binding to binary monolayers composed of 1,2-di-O-hexadecyl-sn-glycerol and derived glycolipids with the mannose moieties. The glycolipid...

The role of the two galactose binding sites of ricin B chain in ricin toxicity was evaluated by studying a series of ricin point mutants. Wild-type (WT) ricin and three ricin B chain point mutants having mutations in either 1) the first galactose binding domain (site 1 mutant, Met in place of Lys-40 and Gly in place of Asn-46), 2) the second galactose binding domain (site 2 mutant, Gly in place...

C1 esterase inhibitor (C1INH) is known to exert its inhibitory effect by binding several target proteases of the contact and complement systems. One C1INH’s targets comprise mannose-binding lectin (MBL), a critical player in post-stroke pathophysiology. We therefore explored effects recombinant human (rh) plasma derived (pd) C1INH C57BL/6J mice subjected transient occlusion middle cerebral arte...

The thioctic acid amides of 2'-aminoethyl alpha-d-mannopyranoside and 2'-aminoethyl alpha-1,3-d-mannopyranosyl (alpha-1,6-d-mannopyranosyl)-alpha-d-mannopyranoside presented on both planar and nanoparticle gold surfaces give higher specific and lower non-specific protein binding than the related 2'-thioethyl glycosides.

Mannose-binding proteins like the macrophage mannose receptor (MR), the dendritic cell-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN) and mannose-binding lectin (MBL) play crucial roles in both innate and adaptive immune responses. Immunoglobulin fusion proteins of the P-selectin glycoprotein ligand-1 (PSGL-1/mIgG(2b)) carrying mostly O-glycans and, as a control, the...