Thioesterase II, the key enzyme which regulates the production of medium-chain fatty acids by the mammary fatty acid synthetase, is expressed specifically in epithelial cells of the rat mammary gland, regardless of their state of differentiation, and we consider the enzyme to be a reliable marker for this cell type. The objective of this study was to determine whether this enzyme is expressed u...

We have previously demonstrated that (guanine-2-)-methyltransferase activity in extracts from 9,10-dimethyl-1,2-benzanthracene-induced rat mammary tumors differs from that of nonneoplastic mammary tissue. In this report, we explore further the nature of these differences by purification and characterization of the two major transfer RNA (tRNA) (guanine-2-)-methyltransferases from transplantable...

Cancer is the one of the main causes of death in cats and dogs, while it is the second most cause of deaths in humans. In third world countries, less attention is paid to the health of pets. The genes (COX-2 and BRCA1) are selected to study the mutation and gene expressional profiling (qPCR, Real Time PCR) in mammary tumor of cats. Being mammals, cats are studied as a model as they may suffer f...

Different types of non-invasive epithelial mammary lesions are associated to a risk of developing breast cancer in the human and canine species. The process of malignant neoplasm progression is observed in canine mammary tumors, where the development of carcinomatous foci (carcinomas in situ) may result in the formation of invasive carcinomas associated to these tumors (Bertagnolli et al., 2009...

THE ROLE OF THE SUPRMAM1 LOCUS IN RESPONSES TO IONIZING RADIATION AND SUSCEPTIBILITY TO MAMMARY TUMORS MAY 2011 NICHOLAS B. GRINER, B.S., UNIVERSITY OF WASHINGTON Ph.D., UNIVERSITY OF MASSACHUSETTTS, AMHERST Directed by: Professor D. Joseph Jerry Loss of p53 function can lead to a variety of cancers, including breast cancer. Mice heterozygous for the p53 gene (designated Trp53) develop spontane...

The role of homozygous ataxia telangiectasia mutated (ATM) mutations in familial and sporadic forms of cancer is well established, but the contribution of ATM heterozygosity to mammary gland and other cancers has been controversial. To test the effect of Atm heterozygosity on mammary gland cancer, mice with complete loss of exon 11 of Brca1 specifically in mammary epithelium (Brca1-MG-Deltaex11...

The human ERBB2 proto-oncogene is widely considered a key gene involved in human breast cancer onset and progression. Among spontaneous tumors, mammary tumors are the most frequent cause of cancer death in cats and second most frequent in humans. In fact, naturally occurring tumors in domestic animals, more particularly cat mammary tumors, have been proposed as a good model for human breast can...

INTRODUCTION Investigations in this laboratory on the carcino genic action of 2-acetylaminofluorene have re vealed that the type of diet fed with the carcinogen can influence the incidence and types of tumors in duced in rats. Female rats of the Alabama Experi ment Station (AES) strain uniformly developed mammary tumors in about 4 months when they were fed this carcinogen on a semi-purified die...

Dose-response relationships for the induction of mammary tumors by a single i.p. injection of 1-methyl-l-nitrosourea (MNU) were studied. Groups of 30 female Sprague-Dawley rats were given i.p. injections of 50, 37.5, 25, 12.5, or 0 mg MNU/kg body weight at 50 days of age. Animals »erepalpated for tumor detection twice weekly throughout a 28-week observation period. Administration of MNU i.p. c...

BACKGROUND A somatic deletion at the proximal end of canine chromosome 27 (CFA27) was recently reported in 50% of malignant mammary tumors. This region harbours the tumor suppressor gene prefoldin subunit 5 (PFDN5) and the deletion correlated with a higher Ki-67 score. PFDN5 has been described to repress c-MYC and is, therefore, a candidate tumor-suppressor and cancer-driver gene in canine mamm...