1. Using a double-lumen tube perfusion technique in vivo, the absorption rates of glucose from a glucose (200 mm011-~) and from a maltose (100 mmol 1-I) solution were measured in the proximal jejunum of six Zambian African adults. 2. In all of the subjects the rate of glucose absorption from the maltose solution was greater than that from the glucose solution. The difference between the mean ra...

Bacteria with strongly depressed phosphoglucomutase (EC 2.7.5.1) activity are found among the mutants of Escherichia coli which, when grown on maltose, accumulate sufficient amylose to be detectable by iodine staining. These pgm mutants grow poorly on galactose but also accumulate amylose on this carbon source. Growth on lactose does not produce high amylose but, instead, results in the inducti...

We describe an iterative approach for creating protein switches involving the in vitro recombination of two nonhomologous genes. We demonstrate this approach by recombining the genes coding for TEM1 beta-lactamase (BLA) and the Escherichia coli maltose binding protein (MBP) to create a family of MBP-BLA hybrids in which maltose is a positive or negative effector of beta-lactam hydrolysis. Some ...

Aims: The Nova StatStrip blood glucose monitoring system was evaluated for precision, accuracy, and interferences from haematocrit and maltose. It was also compared against three other meters and two reference methods. Methods: Heparinised whole blood samples were analysed on the meters. These results were compared with whole blood samples analysed on the Radiometer ABL835 for the interference ...

In this work 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G) was synthesized by Cyclodextrin glucanotransferase (CGTase) from Bacillus sp. SK 13.002 with L-ascorbic acid (AA) as an acceptor and maltose as a glycosyl donor. AA-2G production was analyzed by HPLC and was confirmed by LC/MS results. The reaction parameters, such as pH (4.0-9.0), temperature (25-50°C), time (0-30 h), substrate ratios...

The utilization of sugars, for example lactose or maltose, can take place in E8cherichia coli only in the presence of two catalytic systems, namely permease and enzymes catalysing the breakdown of the sugars within the cell (Cohen & Monod, 1957). The present paper reports mutants that are not capable of utilizing maltose, although amylomaltase is present in the cell. The results, however, indic...

Glycoside linkage (cellobiose versus maltose) dramatically influenced bioenergetics to different extents and by different mechanisms in the hyperthermophilic archaeon Pyrococcus furiosus when it was grown in continuous culture at a dilution rate of 0.45 h(-1) at 90 degrees C. In the absence of S(0), cellobiose-grown cells generated twice as much protein and had 50%-higher specific H(2) generati...

The hyperthermophilic archaeon Thermococcus hydrothermalis was cultivated in continuous culture in a gas-lift bioreactor in the absence of elemental sulphur on both proteinaceous and maltose-containing media. Optimal conditions (pH, temperature and gas flow rate), determined on complex media that yielded maximal growth rate and maximal steady state cell density, were obtained at 80 degrees C, p...

Riboflavinyl glycosides were synthesized enzymatically using amyloglucosidase isolated from Rhizopus mold and βglucosidase from sweet almond to produce more water soluble riboflavinyl derivatives. The reaction conditions were optimized in terms of incubation period, pH, buffer, enzyme and substrate concentrations. With D-glucose, D-galactose, Dmannose, D-ribose, sucrose, maltose and lactose, bo...