conclusions within the limitations of this study, disinfectants were able to completely eliminate microorganisms carried by the alginate impressions. however, more efficient disinfection methods are needed to eliminate the risk of cross contamination by condensational silicone impressions. background dental impressions often carry microorganisms that may cause cross-infection from patients to d...

Nalidixic acid (nal) susceptibility of clinical isolates of Serratia marcescens from urinary tract infections, wounds, and blood was determined by the standard disk method, and results were compared with results obtained by a standardized tube dilution procedure using five different broth media. An attempt was made to approximate in vivo conditions by using urine as a test medium and correlatin...

OBJECTIVES Since biofilm formation is the hallmark of Enterococcus faecalis isolates, the aim of this study was to quantify biofilm formation in the presence of subinhibitory concentrations of tigecycline. METHODS Interference of tigecycline on biofilm formation was spectrophotometrically quantified using 20 biofilm-producing E. faecalis isolates with tigecycline MICs of 0.12 (8 strains) or 0...

RAW264.7 (ATCC TIB-71) cell line, Shigella sonnei (S. sonnei; ATCC 29930), Staphylococcus aureus (S. aureus; ATCC 25923), and Listeria monocytogenes (L. monocytogenes; ATCC 19117) were purchased from America Type Culture Collection (ATCC; Manassas, VA). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle’s medium (DMEM) with high glucose were obtained from Thermo Fisher Scientific (South Log...

Resistance to antimicrobial agents is responsible for major social and economic losses. The World Health Organization estimated 700,000 global deaths a year due resistance. use of layered double hydroxides (LDHs) as antibacterial materials could present way reduce the risk bacterial infections resistance, by partial release metallic ions in aqueous dispersion. dissolution different synthetic LD...

Six Shiga toxin-producing Escherichia coli (STEC) serogroups, which include O26, O45, O103, O111, O121, and O145, are responsible for the majority of non-O157 STEC infections in the United States, representing a growing public health concern. Cattle and other ruminants are reservoirs for these pathogens; thus, food of bovine origin may be a vehicle for infection with non-O157 STEC. Methods for ...

  Escheric hia coli O157:H7 lives in the intestines of healthy cattle, and can contaminate meat during slaughtering pr actices . Detection of the low infectious dosage of bacterium requires a sensitive method. We developed polymerase chain reaction (PCR) assays to detect the gene Stx2 irrespective of the bacterial serotype. In this study, the detection limit of the PCR protocol in detecting Stx...