نتایج جستجو برای: m20 primer with 20 bands
تعداد نتایج: 9375849 فیلتر نتایج به سال:
We have developed a simplified procedure for the ligation-mediated polymerase chain reaction (LMPCR) using Thermococcus litoralis DNA polymerase (Vent DNA polymerase). We show that Vent DNA polymerase produces correct, blunt-ended primer extension products with substantially higher efficiency than Thermus aquaticus (Taq) DNA polymerase or modified T7 DNA polymerase (Sequenase). This difference ...
The eight SSR′s and 15 RAPD′s primers were used to investigate of genetic diversity and relationship between 20 winter rapeseed genotype. Totally 164 bands were detected from Which 154 were polymorphic. The RAPD primers namely Oligo-670 with 16 bands showed the highest number of detected bands. The mount of Nei′s, Shanon and PIC index for Na12-B02, Ni4-B03, OL10-G04, Oligo-543 and Oligo-670 ind...
Hydatidosis is a disease caused by infection with the metacestode stage of the dog tapeworm,Echinococcus granulosus. This is recognised as one of the world’s most important zoonoses, affecting bothhumans and their domestic animals. In the current study, 20 sheep cystic livers or lungs were collected.Hydatid fluid and protoscolices were isolated and the soluble protein was prepared. Polypeptide ...
RAPD markers were used to reveal the genetic diversity of the genus Camptotheca. Three primers (OPA02, OPA03, and OPA04), generating 44 polymorphic bands using randomly amplified polymorphic DNA (RAPD) markers, were able to discriminate among 25 Camptotheca populations. The band size varied from 268-4,411bp, with an average of 15 bands/primer. Of these populations, cultivars ‘Katie’ (KT) and ‘A...
The genetic diversity of 20 Iranian populations of Bunium persicum has been evaluated with random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) markers. Fresh leaves of seedlings from each population were used for genomic DNA extraction. Analysis of banding patterns of 15 RAPD primers and 17 AFLP primer combinations, revealed 192 (86%) and 228 (75%) polymorp...
Abstract Wild melon (Cucumis melo var. agrestis) commonly known as African melon grows in tropical and semi tropical regions of the world. It also known as a valuable medicinal plant has numerous pharmaceutical properties and is considered as a valuable germplasm in cultivated melons breeding. In the present study, genetic variation of sixteen wild melons, collected from Mazandaran provin...
Southern hybridization analysis of the MASP1 gene using an intron-specific probe detected a single band. An exon-specific probe detected several bands. PCR of genomic DNA using several exon-specific primer sets of MASP1 produced short and long products. Sequence of the shorter products corresponded to the processed pseudogene of MASP1. By fluorescence in situ hybridization, this pseudogene (MAS...
A total of nine Trichinella nativa isolates were compared by amplified fragment length polymorphism (AFLP). Four hundred nanograms of genomic DNA from a pool of 10--20 larvae were digested using HindIII and MseI restriction endonucleases. Of the 16 primer combinations initially tested, Hind-C and Mse-C primers showed rich polymorphism with approximately 40--90 bands in the range of 30--270 bp. ...
Collection and conservation of germplasm, particularly for landraces of important agronomically species and organizing their information, cause to continuity of their usefulness and reduce the risk of their genetic erosion. In this experiment, 10 Iranian wheat landraces along with the Chinese Spring cultivar, as control, were used to assess inter and intra populations variation by using ISSR mo...
Three Iranian native strains and three Japanese commercial lines of the silkworm Bombyx mori were analyzed using amplified fragment length polymorphism (AFLP) markers. A set of ten PstI/TaqI primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. Estimates of Nei’s gene diversity for all loci in individual strains and commercial lines indicated a higher degre...
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