نتایج جستجو برای: m pcr
تعداد نتایج: 703897 فیلتر نتایج به سال:
BACKGROUND Despite the many advantages of real-time fluorescence reverse transcription-PCR (RT-PCR) as a quantitative analytical tool, simultaneous quantification of target and reference templates within one reaction has not been reported. We developed such an assay with an internal reference template. METHODS For quantification of target and reference sequences, we used two fluorescent probe...
The dromedary camels are essential because of their multiple uses for transport, wool, meat, and milk production. Camel’s is considered exceptional nutrition, so it therapeutic high nutrition. Mastitis may affect camel’s production, cause significant economic loss, associated with zoonotic disease. study aimed to detect the prevalence Mycoplasma spp. especially M. bovis in using polymerase chai...
Background & Objectives: Chronic obstructive pulmonary disease (COPD) involves the severe obstruction of the airways. Polymerase chain reaction (PCR) is a method with high sensitivity, specificity, accuracy and time-efficiency, which could be used for the identification of different pathogens, especially Mycoplasma pneumonia. This study was performed for the molecular detection of M. pneumonia ...
Mycoplasma genitalium is now recognized as a possible cause of several idiopathic sexually transmitted disease (STD) syndromes. However, due to the difficulty of culture of this fastidious bacterium, nucleic acid amplification tests (NAATs) are necessary for its detection in patient specimens. In the current study we compared a newly developed research-only transcription-mediated amplification ...
The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhoea. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, during the autumn months of 2005, a total of 100 samples from poultry carcasses, representing 20 broiler flocks were obtained by rinse test, after the chilling stage ...
In the present investigation we developed a method for the detection of Mycoplasma hyopneumoniae in bronchoalveolar lavage fluid (BALF) of pigs by PCR with a primer pair flanking a DNA fragment of 853 bp specific for M. hyopneumoniae. Several methods were tested to eliminate the amplification inhibitors present in BALFs. The best results were obtained by the extraction of the DNA from the BALFs...
Background: Paratuberculosis and tuberculosis (TB) caused by Mycobacterium avium paratuberculosis (MAP) and Mycobacterium tuberculosis complex (MTC), respectively are economically important, chronic debilitating diseases affecting the dairy herds and are also potential zoonotic threats. Aims: Differential diagnosis of paratuberculosis and TB i...
background: dermatophytes are the most common causative agents of superficial mycoses. species identification of these fungi is important from therapeutic and epidemiological point of wive. traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstanc...
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