enzymatic hydrolysis of yellowfin tuna visceral protein with neutrase has been carried out by response surface methodology using factorial design. peptide chain length was estimated as the response surface to the hydrolysis conditions (enzyme activity, reaction temperature, and reaction time). the tuna visceral protein hydrolysate had relatively high protein (74.56%) and low lipid content (1.86...

Lysine or Mg2+ alone at high concentrations brings about partial dimerization of the lysine-sensitive aspartokinase of Escherichia coli B. The native enzyme, which has a molecular weight of 75,300 and consists of 2 subunits, responds to a combination of lysine and Mg 2+ at much lower concentrations by dimerizing to a tetrameric molecule with a molecular weight approaching 150,000. This dimeriza...

S-2-Aminoethyl cysteine (AEC) reduced both growth rate and final growth level of Serratia marcescens Sr41. The growth inhibition was completely reversed by lysine. AEC inhibited the activity of lysine-sensitive aspartokinase to a lesser extent than lysine. The AEC addition to the medium lowered not only the level of lysine-sensite aspartokinase but also those of homoserine dehydrogenase and thr...

This study aimed to evaluate the yields of by-products and residues morphometry juvenile tambaqui Colossoma macropomum undernutrition with different levels lysine. Diets were elaborated containing 6.60, 9.72, 12.84, 15.96, 19.08, 22.20 g kg -1 total lysine, these fed fish distributed in 18 tanks (310 L each one) for 90 days. Morphometric analysis, measurements whole fish, by-products, taken com...

In the pharmaceutical industry, cryoprotectants are added to buffer formulations protect active ingredient from freeze- and thaw damage. We investigated freezing thawing of aqueous sodium citrate with various cryoprotectants, specifically amino acids (cysteine, histidine, arginine, proline lysine), disaccharides (trehalose sucrose), polyhydric alcohols (glycerol mannitol) surfactants (polysorba...

conclusions it was shown that the removal efficiency of nitrate was directly proportional to the amount of chaff, temperature, and contact time but inversely to the ph. this study showed that nitrate removal by chaff is a promising technique. results the result of this study revealed that chaff as an absorbent could remove nitrate from solutions, and the efficiency of adsorption increased as co...

background: as fluoride concentration in drinking water is one of the effective parameters in human health, finding the ways to remove excess amount of fluoride is very important in water supply projects. this study aimed to evaluate mendon bauxite mine in the removal of fluoride from drinking water. methods: this experimental study was conducted from may to october 2013 on synthetic and actual...

Maize seed storage proteins are a major source of human and livestock consumption. However, these proteins have poor nutritional value, because they are deficient in lysine and tryptophan. Much research has been done to elevate the lysine content by reducing zein content or regulating the activities of key enzymes in lysine metabolism. Using the naturally lysine-rich protein genes, sb401 and SB...

Salmonella enterica serovar Enteritidis is one of the leading causes of food-borne diseases in Japan. Typically, Salmonella spp. test positive for lysine-decarboxylase. However, the number of isolates of serovar Enteritidis without lysine-decarboxylase activity increased in Japan in 2003. Among 109 strains from distinct outbreaks, 10 lacked lysine-decarboxylase activity. Nine of the ten lysine-...

one experiment was conducted with a total of 2000 broiler breeders (lohman strain) at 74 weeks of age, which lasted for 10 weeks. birds were randomly assigned to two dietary treatments varying in dietary crude protein (cp). the reduced-cp diet had dietary cp level approximately 1.5 percentage units lower than the control (15.5 vs 14.0%). by supplementing 0.13 and 0.1% of dl-methionine and l-hcl...