The capacity of various mycoplasma strains and species to induce lymphocyte transformation in vitro was studied. Of six strains of Mycoplasma pulmonis studied, five displayed mitogenic activity with rat lymphocytes. Among those M. pulmonis strains, our MP15 isolate and the Negroni strain exhibited particularly potent mitogenic capacity. The murine mycoplasmas M. neurolyticum and M. arthritidis ...

The classification of the acute leukemias depends mainly on the morphologic and cytochemical evaluation of the blast forms. One of the main accepted morphologic criteria in the differentiation between acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML) is the absence of granules in the blast cells of ALL. We evaluated a patient with ALL in whom granules were present in the ...

1. Beedassy A, Topolsky D, Styler M, Crilley P. Extramedullary blast crisis in a patient with chronic myeloid leukemia in complete cytogenetic and molecular remission on interferon alfa therapy. Leukemia 2000; 24: 733-735. 2. Carlson NL, Erichson G, Lissel L. Simultaneous meningeal and testicular lymphoblastic transformation of Philadelphia positive CML in a three year old. Anticancer Res 1990;...

A patient with chronic myeloid leukaemia was treated with interferon without using conventional cytotoxic agents. Bone marrow necrosis developed at the onset of blast transformation. It is suggested that cytotoxic drugs should be given before treatment with interferon for chronic myeloid leukaemia. Cytotoxic drugs may also be needed to prevent rapid bone marrow growth once interferon has been w...

We studied the nature of blast cells in 41 patients with acute leukemia following a previous primary myelodysplastic syndrome (MDS) by a combined multiparameter analysis including morphologic, immunophenotypic, and molecular genetic (Igs, T-cell receptor (TCR)-beta, -gamma, and -delta and the major breakpoint cluster region [M-bcr]) investigations. In addition, the clinical and hematologic char...

Antibody-secreting hybridomas were produced by fusion of P3-NS1/1Ag--4-1 mouse plasmacytoma cells with splenocytes from a mouse immunized with HL-60 human promyelocytic leukemia cells. A cloned hybridoma cell line was identified that secreted antibody against a cell surface antigen expressed on all normal human peripheral blood granulocytes, on bone marrow granulocytic precursor cells, and on b...