Methods to study gene expression in live cells over time have been limited. One known method is the luciferase assay, which measures the luminescence of luciferase by coupling its expression to the promoter of a gene under study. This luminescence in cells can be measured over time by a luminometer. One major drawback of the luminometer, however, is that it can only measure the luminescence of ...

Intensity of light emission by luminescent bacteria in response to UV irradiation and chemical mutagens was tested. We demonstrated that luminescence of six strains of marine bacteria (belonging to four species: Photobacterium leiognathi, P. phosphoreum, Vibrio fischeri and V. harveyi) is significantly increased by UV irradiation relatively shortly after dilution of cultures. Such a stimulation...

Lanthanide-doped upconversion (UC) nanoparticles have shown considerable promise in biological labeling, imaging, and therapeutics. However, although current synthetic approaches allow for preparation of ultrasmall UC nanoparticles with precise control over particle morphology and emission color, smaller nanoparticles come at the expense of weaker emissions, which is a constraint that is practi...

We present the results of our time-resolved measurements of hot-carrier luminescence from passively quenched Geiger-mode avalanche photodiodes. In time-correlated photon-counting (TCPC) experiments, hot-carrier luminescence interferes overwhelmingly with the coincidence spectrum, which results in artifacts. This potential problem should be taken into account in setting up TCPC experiments.

The first Eu(3+) chelate-based luminescent probe specific for nitric oxide (NO) has been designed and synthesized for highly sensitive and selective time-gated luminescence detection of NO. Based on the probe, a time-gated luminescence imaging technique was developed for imaging the endogenous NO production in living plant cells/tissues.

We discovered that positively charged terbium complexes bearing 1,4,7,10-tetraazacyclododecane functionalized with amide ligands are highly sensitive to dynamic luminescence quenching by NAD(P)H. We exploited this phenomenon to establish a general time-resolved luminescence-based assay platform for sensitive detection of NAD(P)H-dependent enzyme activities.

We demonstrate the first example of exfoliated layered europium hydroxide (LEuH) composites with a fluorescent molecule that exhibit blue luminescence. Co-quenching in the solid state, blue emission (440 nm) in formamide, and green emission (514 nm) in water-formamide were found, for which delamination and swollen states account for the versatile luminescence behaviors.

We report an approach to enhance simultaneously luminescence and SERS signals with a single excitation wavelength by co-encapsulating silicon quantum dots and Au-Ag alloy nanoparticles encoded with Raman reporter molecules inside polymeric nanoparticles. The SERS-luminescence enhancement exploits the large Stokes shift of silicon quantum dots, which allows 'room' for the display of a Raman spec...

Figure 1. Millipede luminescence and predation. (A) Motyxia sequoiae photographed in natural light, and (B) entirely with light from bioluminescence; (C) Mean proportion of millipedes attacked versus luminescence. Number of individuals attacked above bars; (D) Rodent incisor marks in clay millipede; (E) Live millipede (arrow) with anterior segments 1–14 missing after predator attack. Biolumines...

Gold nanoparticles are efficiently labelled with a luminescent ruthenium complex, producing 13 and 100 nm diameter, monodisperse red-emissive imaging probes with luminescence lifetimes prolonged over the molecular unit. Single, 100 nm particles are observed in whole cell luminescence imaging which reveals their biomolecular association with chromatin in the nucleus of cancer cells.