نتایج جستجو برای: luciferase

تعداد نتایج: 14283  

2004
Martha C. Alvarado Laura M. Zsigmond Izabella Kovács Ágnes Cséplö László M. Szabados

To monitor the expression of T-DNA-tagged plant genes in vivo, a collection of 20,261 transgenic lines of Arabidopsis (Columbia-0) were generated with the promoter trap vector pTluc, which carries a promoterless firefly luc (luciferase) reporter gene linked to the right T-DNA border. By detection of bioluminescence in 3-week-old seedlings, 753 lines were identified showing constitutive, organ-s...

Journal: :The Journal of clinical investigation 2013
Jennifer R Levy Kevin P Campbell

Muscular dystrophies are characterized by progressive muscle weakness and wasting. Among the key obstacles to the development of therapies is the absence of an assay to monitor disease progression in live animals. In this issue of the JCI, Maguire and colleagues use noninvasive bioluminescence imaging to monitor luciferase activity in mice expressing an inducible luciferase reporter gene in sat...

Journal: :Cancer research 2007
Robert U Svensson J Matthew Barnes Oskar W Rokhlin Michael B Cohen Michael D Henry

Bioluminescence imaging is widely used to evaluate tumor growth and response to therapy in living animals. In cells expressing luciferase under the control of a constitutive promoter, light output in part depends on viable cell number, so that changes in bioluminescence intensity may be correlated with changes in viable tumor mass over time. We have found that treatment of cancer cell lines exp...

2006
Xiu Jun Wang John D. Hayes Roland Wolf

The NF-E2 p45-related factor 2 (Nrf2) regulates cytoprotective genes that contain an antioxidant response element (ARE) in their promoters. To investigate whether anticancer drugs can induce ARE-driven gene expression, we have developed a stable human mammary MCF7-derived reporter cell line called AREc32, which contains a luciferase gene construct controlled by eight copies of the cis-element. ...

Journal: :Small 2021

Artificial cells (ACs) aim to mimic selected structural and functional features of mammalian cells. In this context, energy generation is an important challenge be addressed when self-sustained systems are desired. Here, mitochondria isolated from HepG2 employed as natural subunits that facilitate chemically driven adenosine triphosphate (ATP) synthesis. The successful isolation confirmed by mo...

Journal: :Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research 2000
L Farhana M Boyanapalli S H Tschang R J Sun C K Hsu Y X Zhang J A Fontana A K Rishi

Signal transduction via modulation of phosphorylation after selective inhibition of protein phosphatase (PP) 1 and/or PP2A appears to play a role in okadaic acid (OA)-mediated effects. Treatment of several estrogen receptor-negative human breast carcinoma (HBC) cells with 100 nM OA resulted in induction of c-fos, c-myc, and cyclin-dependent kinase inhibitor p21WAF1/CIP1 genes. Transfections of ...

Journal: :Bioscience, biotechnology, and biochemistry 2013
Yuki Hamamoto Takuji Tsukiyama Yoshihiro Yoshikake Masayoshi Teraishi Yutaka Okumoto Takatoshi Tanisaka

We describe a transient dual-luciferase assay combined with a glucocorticoid-inducible system for rice protoplasts. Luciferase genes were efficiently induced by adding 0.1 µM of dexamethasone to the protoplast suspension, the activity of the luciferases reaching a maximum 6 h after induction. This assay system is applicable to studying the translation efficiency of rice by using the luciferase ...

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