This series presents research findings based either directly on data from the German SocioEconomic Panel study (SOEP) or using SOEP data as part of an internationally comparable data set (e.g. CNEF, ECHP, LIS, LWS, CHER/PACO). SOEP is a truly multidisciplinary household panel study covering a wide range of social and The decision to publish a submission in SOEPpapers is made by a board of edito...

RSK2 is a Ser/Thr kinase acting in the Ras/MAPK pathway. Rsk2 gene deficiency leads to the Coffin-Lowry Syndrome, notably characterized by cognitive deficits. We found that mrsk2 knockout mice are unable to associate an aversive stimulus with context in a lithium-induced conditioned place aversion task requiring both high-order cognition and emotional processing. Virally mediated shRNA-RSK2 kno...

In this study we compared techniques for the estimation of protein for studies of marine herbivory. As an example we use gut fluid from the temperate marine herbivorous fish Kyphosus sydneyanus. There were two components to this work: (1) to compare the ability of spectrophotometric methods to estimate protein in gut fluid; and (2) to determine the effect of freezing digesta samples on the extr...

Biochemical analysis of proteins relies on accurate quantitation of protein concentration. This unit describes how to perform commonly used protein assays, e.g., Lowry, Bradford, BCA, and UV spectroscopic protein assays. The primary focus of the unit is assay selection, emphasizing sample and buffer compatibility. Protein assay standard curves and data processing fundamentals are discussed in d...

A network-based analysis of systemic inflammation in humans. Nature. 2005 Aug 31 as doi: 10.1038/nature03985. Steve E. Calvano, Wenzhong Xiao, Daniel R. Richards, Ramon M. Felciano, Henry V. Baker, Raymond J. Cho, Richard O. Chen, Bernard H. Brownstein, J. Perren Cobb, S. Kevin Tschoeke, Carol Miller-Graziano, Lyle L. Moldawer, Michael N. Mindrinos, Ronald W. Davis, Ronald G. Tompkins, Stephen ...

Recent evidence that histamine is present in basophilic kucocytes (Graham, Wheelwright, Parish, Marks and Lowry, 1952; Code and Mitchell, 1954) as well as in tissue mast cells (Riley and West, 1953) has created fresh interest in these cells. Knowledge of variations in the number of crulating basophils is scanty owing to the inaccuracy of smear techniques, but the introduction of a chamber-count...

A rapid and sensitive method for determining protein concentrations using fluorescamine has been characterized for use in the analysis of intact lipoproteins. It was shown that there is no interference with the assay due to the presence of lipid-associated turbidity or primary amine content. The assay was shown to be sensitive to as little as 0.3 p g of lipoprotein and to yield similar results ...

Published jointly with Critical Care Medicine Conference Participants: Mitchell M. Levy (Co-Chair), Graham Ramsay (Co-Chair), Edward Abraham, Derek Angus, Robert Balk, Gordon Bernard, Julian Bion, Joseph Carcillo, Jean M. Carlet, Jonathan Cohen, Deborah Cook, Jean-François Dhainaut, Tim Evans, Mitchell P. Fink, Donald E. Fry, Herwig Gerlach, Steve Lowry, Mark A. Malangoni, John C. Marshall, Geo...

The primary scientific objective of this research is to develop GPS sounding techniques for ground based atmospheric refractivity sensing. Atmospheric profiling with GPS from space has been demonstrated (e.g. Rocken et al., 1997). Ground based receivers have been used to determine integrated atmospheric water vapor above a site, but profiling techniques with ground-based GPS observations are st...