نتایج جستجو برای: lipofection
تعداد نتایج: 251 فیلتر نتایج به سال:
It is generally expected that the kinetics of reactions inside living cells differs from the situation in bulk solutions. Macromolecular crowding and specific binding interactions could change the diffusion properties and the availability of free molecules. Their impact on reaction kinetics in the relevant context of living cells is still elusive, mainly because the difficulty of capturing fast...
BACKGROUND Restenosis after percutaneous coronary intervention remains a serious clinical problem. Progress in local gene therapy to prevent restenosis has been hindered by concerns over the safety and efficacy of viral vectors and the limited efficiency of nonviral techniques. This study investigates the use of adjunctive ultrasound to enhance nonviral gene delivery. METHODS AND RESULTS Cult...
Over the past two decades, researchers have struggled to efficiently express foreign DNA in primary macrophages, impeding research progress. The applications of lipofection, electroporation, microinjection, and viral-mediated transfer typically result in disruptions in macrophage differentiation and function, low expression levels of exogenous proteins, limited efficiency and high cell mortalit...
From the viewpoint of safety, non-viral vector systems represent an attractive gene delivery system for gene therapy. However, the transfection efficiency of non-viral vectors in vivo is generally very low. Previously, it was reported that microbubbles, utilized as imaging agents for diagnostic echocardiography, could promote gene delivery into cells when combined with ultrasound exposure. We t...
سابقه و هدف: هیدروکسی اوره (HU) دارویی است که می تواند منجر به القای ژن- g گلوبین به منظور درمان - bتالاسمی گردد. در این مطالعه اثر غلظت های مختلف این دارو بر میزان القای ژن - gگلوبین در سلول های K562 بررسی و غلظت بهینه جهت القا ژن - gگلوبین در این سلول ها تعیین و هم چنین اثر مهاری siRNA بر ژن کاندیدای مسیر سیگنالی HU در سلول های K562 بررسی شد.روش بررسی: در این مطالعه تجربی، سلول های K562 با غل...
background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...
Introduction Modulating gene expression, either by transfection or by RNA interference (RNAi), is a powerful means for studying the functions of essential genes during nervous system development and maintenance. Both approaches depend on the successful delivery of the corresponding nucleic acids into the targeted cells. Current methods for introducing DNA and RNA into cells and tissues include ...
many diseases, has some potential for the treatment of certain types of serious cancer such as lung cancer. During the past 15 years, more than 400 clinical studies in gene therapy have been evaluated and almost 70% of these studies were in the area of cancer gene therapy. However, even though much clinical research has been carried, the validity of this treatment has not been confirmed. The ke...
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