Erucic acid (EA), a fatty acid of 22-carbon chain with one isolated double bond, has broad industrial applications. Currently, erucic acid is isolated from Crucifereae plant oils through steam splitting, namely the Colgate-Emery process, followed by fractional distillation. This two-step process involves drastic conditions, low energy efficiency, and extensive product degradation. Enzymatic app...

Lipases (triacylglycerol acylhydrolase, EC 3.1.1.3), catalyze the hydrolysis of long-chain triglycerides with the formation of diacylglyceride, monoglyceride, glycerol and fatty acids. Lipases are actively used in various industries which include food and diary, pharmaceuticals, organic synthesis and detergent and cosmetics. Thermostable lipases are commercially significant for their potential ...

A new series of 4-aryl-1, 4-dihyropyridines possessing potent calcium channel blocking activity along with good vasodilatory profile by the modified Hantzsch condensation using trifluoroacetic acid as catalyst has been synthesized and reported from our laboratory. The present work describes chemo enzymatic approach for the enantioselective hydrolysis of a 1, 4dihydropyridine skeleton. In this s...

The hydrolysis of triglycerides by grossly normal male human aortas has been studied in vitro. The tissue contains an acid lipase (pH optimum, 5.4) and an alkaline lipase (pH optimum, 8.8). Both lipases catalyze the hydrolysis of saturated triglycerides; the rate decreases with increasing fatty acyl chain from C(10) to C(18). Glycerol trioleate, trilinoleate, and trilinolenate are hydrolyzed at...

Lipases are versatile biocatalysts that are used extensively in detergent and pharmaceutical formulations. Lipases have an immense potential for being employed as industrial catalysts for production of fine chemicals. Their superior value arises from specificity and efficacy as compared to chemical catalysts. However new lipases with properties amenable for application in specific industrial pr...

Fossil fuels are consumed so rapidly that it is expected that the planet resources will be soon exhausted. Therefore, it is imperative to develop alternative and inexpensive new technologies to produce sustainable fuels, for example biodiesel. In addition to hydrolytic and esterification reactions, lipases are capable of performing transesterification reactions useful for the production of biod...

Structured lipids (SL) were synthesized by the acidolysis of borage oil with caprylic acid using lipases. Six commercial lipases from different sources and a novel lipase from Pichia lynferdii NRRL Y-7723 were screened for their acidolysis activities and Lipozyme RM IM and NRRL Y-7723 lipase were selected to synthesize symmetrical SL since recently NRRL Y-7723 lipase was identified as a novel c...

BACKGROUND Preeclampsia (PE) is associated with maternal and neonatal morbidity and mortality. In PE, the physiological hyperlipidaemia of pregnancy is exaggerated. The purpose of this study was to examine the expression of adipose triglyceride lipase (ATGL), hormone sensitive lipase (HSL), lipoprotein lipase (LPL) and endothelial lipase (EL) in pregnancies complicated by PE. METHODS Placenta...

A halotolerant thermostable lipase was purified and characterized from the marine bacterium Oceanobacillus sp. PUMB02. This lipase displayed a high degree of stability over a wide range of conditions including pH, salinity, and temperature. It was optimally active at 30 °C and pH 8.0 respectively and was stable at higher temperatures (50-70 °C) and alkaline pH. The molecular mass of the lipase ...

An antibody raised against purified glyoxysomal lipase (triacylglycerol hydrolase EC 3.1.1.3.) from castor bean (relative molecular weight of 62,000) also binds to a protein with a relative molecular weight of 62,000 in extracts of food reserve tissues from many young oilseed plants. These plants include Brassica napus L., Zea mays L., Arachis hypogaea L., Glycine max L., Gossipium hirsutum L.,...