conclusions high hev seroprevalence (16.7%) was observed among the blood donors in bushehr province. it appears that exposure to hev increases with age; although, more people should be examined. results overall, 105 (16.7%) blood samples were positive for hev-specific-igg antibodies, while 523 (83.8%) were negative. the presence of anti-hev igg was not associated with gender; however, it was co...

background rheumatoid arthritis (ra) is the most common chronic inflammatory disease. the staphylococcal superantigens are considered as the causative agent of ra disease. objectives this study aimed to assess the presence of staphylococcal enterotoxin d in synovial fluid and blood of patients with ra. patients and methods a total of 120 blood and sf samples of patients with ra were studied. ba...

background the etiology of the guillain-barre syndrome (gbs) is not clear; in many cases, gbs is triggered by a bacterial or viral illness. objectives to evaluate the role of active infection with herpesviridae group and its association with this syndrome we have investigated the cerebrospinal fluid (csf) and serum of patients with gbs for detection of these viruses. patients and methods in a pr...

The results of a field survey some potato growing areas in Nineveh Governorate showed the presence Potato virus s PVS, which was detected by Double Antibody Sandwich Enzyme-Linked ImmunoSorbent Assay DAS-ELISA test. This record is first governorate. Through absorbance readings using elisa reader, highest value recorded Sherikhan area samples, amounted to 1.190 nm, while lowest Rabia samples wit...

Objective: The aim of this study was to determine the seroprevalence Toxoplasma gondii (T. gondii) in schizophrenia patients and reveal importance toxoplasmosis as a risk factor these patients. Method: conducted on 190 diagnosed with schizophrenia. Also included control group were 100 healthy individuals without Anti-T. immunoglobulin G (IgG) IgM antibody levels determined via enzyme-linked imm...

A sensitive and reproducible enzyme linked immunosorbent assay (ELISA) has been developed for the detection of ricin. The assay was developed using mouse polyclonal anti-ricin antibodies produced at AMRL and commercially available antibodies. The most efficient ELISA, using a goat anti-ricin antibody as the capture antibody, a rabbit anti-ricin as the second antibody and an alkaline phosphatase...

background: nowadays, chlamydia trachomatis is known as a causative agent of infertility. because of, asymptomatic nature of infection, many may suffer from its lasting complications such as infertility. this study was performed in tehran during april 2007 to april 2008 to compare the prevalence of chlamydia trachomatis infection in fertile and infertile women using elisa and pcr methods. metho...

An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive.

We describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) for the sensitive and rapid detection of antibodies to Ross River virus (RRV) in human sera and known vertebrate host species. This ELISA provides an alternative method for the serodiagnosis of RRV infections.

The reactivities of sera from chronic chagasic patients against the trypomastigote excreted-secreted antigens (TESA) of Trypanosoma cruzi strains with different biodemes were analyzed by TESA-blot and TESA-enzyme-linked immunosorbent assay (ELISA). Although both tests presented high sensitivity and specificity, TESA-ELISA is more appropriate for screening a larger number of samples.