OBJECTIVES To explore the source of the p19 subunit of interleukin-23 (IL-23) in joints with rheumatoid arthritis (RA), the effects of IL-1beta and tumour necrosis factor (TNF)-alpha on IL-23 gene expression in RA fibroblast-like synoviocytes and the effect of IL-23 on proinflammatory cytokines. METHODS Expression of IL-23 p19 in joints was examined by immunohistochemical analysis of patients...

PRP cryopreservation remains a controversial point. Our purpose was to investigate the effect of freezing/thawing on PRP molecule release, and its effects on the metabolism of chondrocytes and synoviocytes. PRP was prepared from 10 volunteers, and a half volume underwent one freezing/thawing cycle. IL-1β, HGF, PDGF AB/BB, TGF-β1, and VEGF were assayed 1 hour and 7 days after activation. Culture...

Eupatilin is the main active component of DA-9601, an extract from Artemisia. Recently, eupatilin was reported to have anti-inflammatory properties. We investigated the anti-arthritic effect of eupatilin in a murine arthritis model and human rheumatoid synoviocytes. DA-9601 was injected into collagen-induced arthritis (CIA) mice. Arthritis score was regularly evaluated. Mouse monocytes were dif...

As a kind of chronic inflammatory diseases, Rheumatoid arthritis (RA) has low cure rate and easy recurrence. It widely reported that abnormal activation mitogen-activated protein kinase (MAPK) nuclear factor kappa-B (NF-κB) signaling pathways are associated with the development RA inflammation. Blocking rheumatoid fibroblast-like synoviocytes (RA-FLS) can delay RA. Ononin is natural isoflavone ...