The present study is the first report on quantitative analysis of secondary metabolites in callus cultures Barleria prionitis L. and comparison with mother plant. Callus was obtained from stem internode (on MS medium 0.5 mg l−1 NAA, BAP 300 ascorbic acid), leaf 1.0 2,4-D, BAP, acid ) explants and, multiplied by subculturing thrice at an interval 25 days. Calli plant counterparts were extracted ...

Himantoglossum affine is a threatened terrestrial orchid. We aimed to optimize asymbiotic seed germination and direct embryogenesis analyze the phytochemical profile physico-biochemical analysis of leaf tuber. The individual use organic nitrogen compounds resulted in higher efficiencies, while shortest times were observed using coconut water plus casein hydrolysate. Plantlets grown on media sup...

Coffea arabica L. is a species of coffee that contribute for more than seventy percent world production. Various attempts have been made to obtain large quantities planting material through propagation in vitro somatic embryogenesis technology. The objective this experiment was evaluate the effect different plant growth regulators (PGRs) on callus induction (indirect embryogenesis) AS2K clone A...

tissue. That is, the dorsal donor graft redirects the fate Vertebrates begin life as a seemingly unpatterned clusof the host’s ventral mesoderm into properly organized ter of cells. Rapidly, however, the shape of an embryo dorsal mesoderm with a notochord and muscle segchanges and begins to acquire an easily recognizable ments. The donor tissue also repatterns the host’s venpattern. This format...

Callus induction and regeneration of fennel from embryo explants were stabilized in the presence of cefotaxime antibiotic and different plant growth regulators (PGRs). The experiments were conducted under a factorial experiment, based on a completely randomized design (CRD). Genotypes; Fasa, Meshkinshar and Hajiabad were applied under different concentration of cefotaxime (0 and 100 mg l-1...

Pure neuroectodermal growth was obtained from the retina of the chicken embryo by removing the pecten before preparation of the cultures. Retinal tissue was cultured either as explants in plasma clots or as isolated cells after trypsinization. Epithelial cells formed sheets on which the other retinal cells grew. Horizontal cells maintained the associational characteristics. Development of photo...