Background: Reactive oxygen species (ROS) is generated by spermatozoa from human (Aitken and Clarkson, 1987; Alvarez et al., 1987), rat (Vernet et al., 2001), mouse (Fisher and Aitken, 1997), rabbit (Holland et al., 1982) and buffalo (Roy and Atreja, 2008). ROS initiates signaling pathways regulating key processes including capacitation (Aitken et al., 1998;Aitken et al., 1996;deLamirande et al...