A G-quadruplex-based, label-free, switch-on fluorescence detection method has been developed for the selective detection of ATP in aqueous solution using crystal violet as a G-quadruplex-selective probe. The assay is highly simple and rapid, and does not require the use of fluorescent labeling.

The title compound, C(8)H(10)N(2)O(2)S, was prepared by the reaction of 2-cyano-3,3-bis-(methyl-sulfan-yl)acrylate and 2-amino-ethanethiol at 350 K. The mol-ecular structure and packing are stabilized by N-H⋯O hydrogen-bond inter-actions. All the non-H atoms are nearly in the same plane with the maximum deviation being 0.08 Å.

We report on what is believed to be the first example of an ultrawide, bandpass filter, based on a high-contrast multicomponent one-dimensional Si photonic crystal (PC). The effect of the disappearance of a limited number of flat stopbands and their replacement with extended passbands is demonstrated over a wide IR range. The passbands obtained exhibit a high transmission of 92% to 96% and a su...

Kennedy and Barbaro (1953) presented data suggesting that crystal violet adsorption values correlated directly with the gram staining character of bacterial cells, and implied that such quantitative dye uptake studies might prove a basis for a much needed quantitative index of gram positivity. Their results are consistent with a popular theory of the mechanism of gram differentiation presented ...

GFP and YFP are fluorescent proteins that are widely used in various cellular assays (1). The 488-nm blue laser, that is standard on virtually all flow cytometers, efficiently excites both proteins. GFP and YFP signals can be discriminated using a customized filter configuration involving a 550/30-nm band pass for YFP, a 510/20-nm band pass filter for GFP, with a 525-nm short-pass dichroic mirr...

In this paper I have chosen some diseases and conditions which can be greatly benefited by the use of ultra-violet light. In writing these notes I have dealt with each subject from three points of view, viz : (1) the rationale of the use of the ultra-violet rays; (2) the available authoritative literature on the subject; and (3) the technique in detail. The following ten conditions will now be ...

Abstract-In-situ chemical polymerization was used to synthesize PPy/ TiO2 nanocomposite. The nanocomposite was characterized by X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Scanning electron microscopy (SEM) and Energy dispersive X-ray spectroscopy (EDX) to ensure the crystallite size, functional groups and morphology of the polymer nanocomposite. The band gap of th...

effectiveness of the ultra-violet dose by the dose-independent factor q. This makes it possible to surmise that in our experiments the killing of the bacteria and the production of the mutants might be due to the same chemical effect produced by the ultra-violet irradiation. 1 Kelner, A., PROC. NATL. ACAD. ScI., 35, 73 (1949). 2 Dulbecco, R., Nature, 163, 949 (1949). 3 Witkin, E. M., Genetics, ...

Ultra violet spectrophotometric estimation of the raltegravir potassium, an integrase inhibitor antiretroviral agent was estimated by Ultra violet absorption maxima method at λmax of 328 nm and UV area under curve method in the wave length range of 323-333 nm. The Beer's law obeyed in the concentration range of 3-55 μg/ml and correlation coefficients were found to be more than 0.996 for both me...

Mutants of Crithidia fasciculata, resistant to Actinomycin D, Tubercidin, Crystal Violet, 6-Azauridine, and 5-Fluorouracil were obtained and characterized. The mutants were stable when maintained under nonselective conditions, and could be grouped in several subclasses on the basis of cross-resistance to other drugs. Actinomycin D- and Crystal Violet-resistant mutants appeared spontaneously whe...