Using catalytic cytochemistry the AC activity was studied during ischemic preconditioning (IP) (5 min occlusion of LAD and 10 min reperfusion) followed by 30 min regional ischemia in isolated Langendorff-perfused rat heart. In controls the specific precipitate of AC reaction was found on the sarcolemma (SL) and the junctional sarcoplasmic reticulum (JSR) of cardiomyocytes. After prolonged ische...

Transient receptor potential melastatin member 4 (TRPM4) encodes a Ca2+-activated, non-selective cation channel that is functionally expressed in several tissues, including the heart. Pathogenic mutants TRPM4 have been reported patients with inherited cardiac diseases, conduction blockage and Brugada syndrome. Heterologous expression of mutant channels cell lines indicates these mutations can l...

The isolated perfused heart system, as originated by Oscar Langendorff more than a century ago, has become a predominant technique in pharmacological and physiological research. The technique allows the examination of cardiac contractile strength (inotropic effects), heart rate (chronotropic effects) and vascular effects without the complications of an intact animal model. From its simple begin...

Objective(s) This study aimed to examine whether acetyl-L-carnitine (ALC) was able to reduce cardiac arrhythmias and infarct size in the ischemic-reperfused isolated rat heart. Materials and Methods The isolated hearts were mounted on a Langendorff apparatus then perfused by a modified Krebs-Henseleit solution during 30 min regional ischemia and 120 min reperfusion (control) or by enriched Kreb...

Monophasic action potential (MAP) can be recorded from the heart surface by optical method based on fluorescence measurement. The motion of isolated heart during experiment caused additional noise in recorded signal. The motion artifact can be eliminated by ratiometric fluorescence emission measurements. This study is based on experiments in which optical MAP measurement is done by single-wavel...

Genetic ablation of GLUT4, the major insulin sensitive transporter, results in insulin resistance without overt diabetes. GLUT4 Null mice exhibit significantly decreased adipose tissue and severe cardiac hypertrophy (2x Control) while maintaining normal whole body glycemia. The hypertrophy seen in Null animals is not due to pressure overload. Histological examination revealed enlarged myocytes ...

The incorporation of [32Plinorganic phosphate into membranous, myofibrillar, and cytosolic proteins was studied in Langendorff-perfused guinea pig hearts treated with phorbol 12myristate 13-acetate (PMA) or 1,2-dioctanoylglycerol (D8G), which are potent activators of protein kinase C. Control hearts were perfused with an inactive phorbol ester (4c-phorbol 12,13-didecanoate), which does not caus...

in this study, we investigated the cardioprotective effects of resveratrol. rats were intraperitoneally administered with resveratrol (25 mg/kg bw) or vehicle (ethanol 10%) for 7 days and their heart subjected to ischemia/reperfusion injury. isolated hearts were langendorff perfused, left ventricular functions as heart rate and developed pressure, as well as, heart antioxidant status were deter...

1. Intracellular Ca(2+) overload has been associated with established atrial arrhythmogenesis. The present experiments went on to correlate acute initiation of atrial arrhythmogenesis in Langendorff-perfused mouse hearts with changes in Ca(2+) homeostasis in isolated atrial myocytes following pharmacological procedures that modified the storage or release of sarcoplasmic reticular (SR) Ca(2+) o...

Transverse tubules (T-tubules) are orderly invaginations of the sarcolemma in mammalian cardiomyocytes. The integrity of T-tubule architecture is critical for cardiac excitation-contraction coupling function. T-tubule remodeling is recognized as a key player in cardiac dysfunction. Early studies on T-tubule structure were based on electron microscopy, which uncovered important information about...