Meat source fraud and adulteration scandals have led to consumer demands for accurate meat identification methods. Nucleotide amplification assays have been proposed as an alternative method to protein-based assays for meat identification. In this study, we designed Loop-mediated isothermal amplification (LAMP) assays targeting species-specific mitochondrial DNA to identify and discriminate eig...

Piglets from birth to wean at 21 days of age were subjected to heat lamps with either constant power output (CO) of 175 W or variable power output (VO) from 175 W at birth to 100 W by weaning time. Each type of heat lamp had either clear or red radiant rays. The effects of lamp output and color on heat lamp usage (HLU) and average daily gain (ADG) of the piglets were examined for a cool air tem...

We developed an enrichment medium for use with the loop-mediated isothermal amplification (LAMP) assay (enrichment media + LAMP assay) to quickly increase a very small number of Vibrio parahaemolyticus cells to the detection limit of the assay. Thirty-nine different enrichment media were prepared based on evaluating 12 potential ingredients. From our assessment of the 39 media, enrichment m...

INTRODUCTION In 1997, a European guideline concerning the viscosity and surface tension of lamp oil was adopted to reduce instances and severity of lamp oil intoxications. In 2005, the Dutch National Poisons Information Centre investigated lamp oil intoxications to determine whether they differed in severity from the intoxications reported before the guideline was adopted. METHODS We compared...

Animal species detection is one of the crucial steps for consumer’s food analysis. In the present study we developed an in-house built loop-mediated isothermal amplification (LAMP) assay for rapid detection of adulterated cow DNA in goat milk/meat samples. The cow milk/tissue DNA in goat milk/meat samples were identified in the developed LAMP assay by either naked eye visualizing with SYBR Gree...

Recently, many studies have demonstrated the significant advantages of loop-mediated isothermal amplification (LAMP) based methods over serological tests and PCR for rapid detection of microbial pathogens. Here, a rapid LAMP assay was developed to detect the hepatitis B virus (HBV) from DNA, and particularly, blood samples from infected patients using a commercially available master mix and por...

Thermal and thermo-mechanical modeling and characterization of solid state lightening (SSL) retrofit LED Lamp are presented in this paper. Paramount importance is to design SSL lamps for reliability, in which thermal and thermo-mechanical aspects are key points. The main goal is to get a precise 3D thermal lamp model for further thermal optimization. Simulations are performed with ANSYS and Cov...

The lifetime of a fluorescent lamp mainly depends on the loss of Ba atoms from its electrode, i.e. the rate of Ba atoms loss controls the lifetime of the lamp. The mechanism of the loss has not been clarified yet sufficiently. We have investigated relationship between electrode temperature distributions and characteristics of Ba atom emission in a fluorescent lamp which are measured by using bl...

A rapid detection assay based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been developed for detecting porcine reproductive and respiratory syndrome virus (PRRSV). The RT-LAMP assay utilized a set of six primers to amplify the open reading frame 6 (ORF6) of the PRRSV. The amplified products were analyzed by agarose gel electrophoresis or visualized by colorimet...

Pneumocystis pneumonia (PCP) is one of the common opportunistic infection among HIV and non-HIV immunocompromised patients. The lack of a rapid and specific diagnostic test necessitates a more reliable laboratory diagnostic test for PCP. In the present study, the loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Pneumocystis jirovecii. 185 clinical respirato...