* Corresponding author Production of β-xylosidase by a cycloheximide and 2deoxy-D-glucose-resistant mutant of Kluyveromyces marxianus PPY125 was studied when cultured on growth media containing galactose, glucose, xylose, cellobiose, sucrose and lactose as carbon sources. Xylose, cellobiose, lactose and sucrose were the key substrates. Both K. marxianus PPY125 and its mutant (M 125) supported m...

Lactose in dairy systems can exist in various crystalline and non-crystalline forms. These forms affect lactose behaviour, particularly in processing and storage of low-water dairy foods. Crystalline -lactose monohydrate and anhydrous -lactose are well-known solid forms of lactose, which are relatively poorly soluble in water. Its occurrence in two anomeric forms, and -lactose, makes its solubi...

Solutions of maltodextrins (MD) with the dextrose equivalent (D.E.) 9-12 and D.E. 23-27 were mixed with lactose solutions at different ratios. Freeze-dried amorphous solids of lactose and lactose-MD were prepared from solutions containing 20% total solids. The ratios of lactose and MD were 90:10, 80:20, and 70:30 (w/w). Crystallization rate of amorphous lactose was followed from changes in wate...

The methods which are used to, show the hydrolytic splitting of lactose and maltose, are not so simple as those which demonstrate the hydrolysis of saccharose and polysaccharides. The reason for this is that in the latter case there is a change from a non-reducing carbohydrate to a reducing sugar, whilst in the former the original material will already reduce cupric hydrate in alkaline solution...

10 This work reports the optimization of a cost-effective and scalable process for 11 the enzymatic synthesis of kojibiose (2-O-α-D-glucopyranosyl-α-D-glucose) from 12 readily available and low-cost substrates such as sucrose and lactose. This 13 biotechnological process is based on the dextransucrase-catalysed initial synthesis of a 14 galactosyl-derivative of kojibiose (4-O-β-D-galactopyranos...

Bifidobacterium longum subsp. infantis is a common member of the intestinal microbiota in breast-fed infants and capable of metabolizing human milk oligosaccharides (HMO). To investigate the bacterial response to different prebiotics, we analyzed both cell wall associated and whole cell proteins in B. infantis. Proteins were identified by LC-MS/MS followed by comparative proteomics to deduce th...

It has been suggested that lactose malabsorption is an important factor in producing the diarrhoea of acute rotavirus infection. Accordingly, the lactose tolerance of gnotobiotic newborn lambs, infected with lamb rotavirus, has been investigated by clinical studies and tissue enzyme assays. Although lactase activity is low in affected areas of the small intestine, rotavirus infected lambs are n...

Skim milk of approximately 3% total protein was ultrafiltered to 16.2% protein and 4.1 % lactose, and then diafiltered to reduce lactose to 2.1% and 0.9%. Total protein con ten t was maintained at approximately 17%. A ponion of the sk im milk was condensed in a rising film evaporator to 14.7% protein and 15.7 % lactose . All concentrates were spray dried at 120 to 125 °C inlet air temperature a...

BACKGROUND symptoms attributed to the lactose intolerance are an important public health issue because of their prevalence and social relevance. Also because they may cause undue rejection of dairy products consume with potential health consequences. Transit time is a putative factor implied in the severity of symptoms associated with lactose. OBJECTIVES to elucidate the relation between oroc...