A total of 10 metallo-β-lactamase-producing isolates of six different species, including Brevundimonas diminuta (n = 3), Rhizobium radiobacter (n = 2), Pseudomonas monteilii (n = 1), Pseudomonas aeruginosa (n = 2), Ochrobactrum anthropi (n = 1), and Enterobacter ludwigii (n = 1), were detected in the sewage water of a hospital. The presence of bla(VIM-13) associated with a Tn1721-class 1 integr...

The antibiotic susceptibilities and capabilities to induce beta-lactamases were studied in multiple Escherichia coli murein (peptidoglycan) hydrolase mutants. E. coli mutants lacking either three amidases, three amidases and one lytic transglycosylase, or six lytic transglycosylases showed higher levels of susceptibility to bacitracin, erythromycin, gallidermin, and vancomycin than the wild typ...

In this study, 78 isolates of Escherichia coli isolated from Korean beef cattle farms were investigated for the production of extended-spectrum β-lactamase (ESBL) and/or AmpC β-lactamase. In the disc diffusion test with ampicillin, amoxicillin, cephalothin, ceftiofur, cefotaxime, ceftazidime, and cefoxitin, 38.5% of the isolates showed resistance to all of ampicillin, amoxicillin, and cephaloth...

Despite the fact that P-lactamases from a range of bacterial species Gram-positive and Gram-negative show evolutionary relatedness, there is no set pattern to the genetic organization that underlies their synthesis and its regulation. Thus, for example, the enzymes of many Gram-positive species are extracellular and inducible, whereas their counterparts in Gram-negative bacteria are often produ...

Despite the fact that P-lactamases from a range of bacterial species Gram-positive and Gram-negative show evolutionary relatedness, there is no set pattern to the genetic organization that underlies their synthesis and its regulation. Thus, for example, the enzymes of many Gram-positive species are extracellular and inducible, whereas their counterparts in Gram-negative bacteria are often produ...

To control the antibiotic resistance epidemic, it is necessary to understand the distribution of genetic material encoding antibiotic resistance in the environment and how anthropogenic inputs, such as wastewater, affect this distribution. Approximately two-thirds of antibiotics administered to humans are -lactams, for which the predominant bacterial resistance mechanism is hydrolysis by -lacta...

OBJECTIVES Acinetobacter baumannii is a significant pathogen in health care settings. In recent years, an increase in carbapenem resistance among A. baumannii due to Ambler class B metallo-beta-lactamases or class D OXA carbapenamases has been reported. In this study we detected the presence of OXA carbapenamases and coproduction of metallo-beta-lactamases (blaVIM and blaIMP ) by phenotypic and...

Epidemiologic surveillance study was conducted in southern Japan to determine the antimicrobial resistance phenotypes and characterize the β-lactamase genes and the plasmids harboring these genes in Salmonella enterica serovar Infantis (S. Infantis) isolates from broilers. Between January, 2007 and December, 2008, a total of 1,472 fecal samples were collected and examined at the Laboratory of V...

Cloned division genes (ftsQ and ftsA) and the gene for beta-lactamase (bla) were transcribed in vivo from a bacteriophage T7 promoter under conditions which blocked the use of other promoters. The different coding regions of single mRNAs were translated with widely different efficiencies, such that the ratio of beta-lactamase production to FtsQ production was about 75:1. The relative rates of t...