Convenient expression systems for efficient heterologous production of different laccases are needed for their characterization and application. The laccase cDNAs lcc1 and lcc2 from Trametes versicolor were expressed in Pichia pastoris and Aspergillus niger under control of their respective glyceraldehyde3-phosphate dehydrogenase promoters and with the native secretion signal directing catalyti...

Nanotechnology has transformed the science behind many biotechnological sectors, and applied bio-catalysis is not exception. In 2017, enzyme industry was valued at more than 7 billion USD projected to 10.5 by 2024. The laccase an oxidoreductase capable of oxidizing phenolic non-phenolic compounds that have been considered essential tool in fields currently known as white biotechnology green che...

High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove from wastewaters, researchers focused on applying immobilized enzymes mild reaction conditions enzyme nontoxicity. Laccases oxidases with wide substrate specificity, capable degradation many different dye types. Laccase Streptomyces cyaneus was expres...

The large family of enzymes, known as polyphenols oxidases, includes laccase. Due to the inclusion a copper atom in their catalytic core, laccases are frequently referred multi-copper oxidases. Laccases versatile enzymes that can catalyze oxidation wide range phenolic and non-phenolic substances. In current study, local strain Aspergillus niger was used for solid-state fermentation produce fung...

In the present study, Plackett–Burman design (PBD) followed by response surface methodology (RSM) was used to optimize the culture medium composition for laccase production from Pleurotus ostreatus IMI 395545. Influence of carbon, nitrogen (sources) and inducer were evaluated by Plackett–Burman design. The factors that induce positive effect on enzyme production were further optimized using cen...

The ability to catalyse an electrode reaction via direct (mediatorless) electron transfer has been demonstrated for a number of redox enzymes. In the case of mediatorless electron transfer, the electron is transferred directly from the electrode to the substrate molecule via the active site of the enzyme, or vice versa. The electron itself is the second substrate for the reaction. An important ...

Iodine is a well known antimicrobial compound. Laccase, an oxidoreductase which couples the one electron oxidation of diverse phenolic and non-phenolic substrates to the reduction of oxygen to water, is capable of oxidizing unreactive iodide to reactive iodine. We have shown previously that laccase-iodide treatment of spruce wood results in a wash-out resistant antimicrobial surface. In this st...

Enzyme cocktails can alter the lignin and hemicellulose content in wood cell walls, improving bleaching process during pulp production offsetting need for toxic chemicals. In this study, brown was biobleached with a mixture of crude fungal extracts rich xylanase laccase, respectively produced from Aspergillus tamarii Kita Trametes versicolor on waste materials. The optimal conditions biobleachi...

Laccase is an enzyme that has potential ability of oxidation. It belongs to those enzymes, which have innate properties of reactive radical production, and its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. Textile, pulp and paper industries discharge a h...

Panaeolus sphinctrinus, Panaeolus papilionaceus, and Coprinus friesii are described as producers of ligninolytic enzymes. P. papilionaceus and P. sphinctrinus both produced a laccase. In addition, P. sphinctrinus produced a manganese peroxidase. C. friesii secreted a laccase and two peroxidases similar to the peroxidase of Coprinus cinereus. The purified laccases and peroxidases were characteri...