The aim of the present study reported here is to examine the production, purification and characterization of the enzyme laccase from the mushroom Hypsizygus ulmarius. The culture conditions were optimized for the maximum production of enzymes with different parameters such as incubation time, carbon, nitrogen sources. The enzyme was purified by ammonium sulphate precipitation and partially cha...

Indigo carmine is a typical recalcitrant dye which is widely used in textile dyeing processes. Laccases are versatile oxidases showing strong ability to eliminate hazardous dyes from wastewater. However, most laccases require the participation of mediators for efficient decolorization of indigo carmine. Here we describe the improvement of the decolorization ability of a bacterial laccase throug...

Reduction-oxidation of native Rhus vernicifem laccase produces specific long-lived spectral changes in the near UV and visible region. These can be attained 1) in aerobic reduction experiments, 2) from partially and fully reduced laccase molecules reacting with 0 2 , 3) by reacting peroxylaccase with 2 reduction eq, and 4) upon reoxidation of fully reduced laccase by 2 mol eq of HzOz. The spect...

Phenoloxidases (POs) play a key role in melanin production, are involved in invertebrate immune mechanisms, and have been detected in different bivalves. Recently, we identified catecholase- and laccase-like PO activities in plasma and haemocyte lysate supernatant (HLS) of the Pacific oyster Crassostrea gigas. To go further in our investigations, the aims of this study were (i) to determine the...

Laccase has huge potential application in all aspects of biotechnology due to the ability laccase oxidize a wide range phenolic and non-phenolic compounds. The future success such applications requires large amounts with low costs. White-rot fungi are important groups production. In this study, three white-rot fungi, Phlebia acerina Han 618, Trametes hirsuta 726, Coriolopsis trogii 751, isolate...

A novel laccase was purified from the white rot fungus, Hohenbuehelia serotina, to investigate the applications of this laccase in the decoloration of various dyes. SDS-PAGE revealed a single band of this laccase corresponding to a molecular weight of approximately 57.8 kDa. The enzyme showed activity towards several substrates, the most sensitive of which was 2,2'-Azinobis-(3-ethylbenzthiazoli...

Enzyme application in biotechnological and environmental processes has had increasing interest due to its efficiency, selectivity and mainly for being environmentally healthful, but these applications require a great volume of enzymes. In this work the effect of different concentrations of ethanol and 2,5-xylidine on growth and production of laccase by Pycnoporus sanguineus was investigated. In...

A laccase-mediated system for denim overdyeing using phenolic compounds was developed. Laccase from ascomycete Myceliophthora thermophila was able to oxidize phenolic compounds such as catechol and catechin and mediate their attachment to denim surfaces. Laccase-generated polymers gave rise to new coloration states from dark brown to green-yellow and replaced dyes in the overdyeing process. Pro...

The heterologous expression of the gene LACP83 (encoding a laccase) from Pleurotus ostreatus in Escherichia coli was characterized. The laccase enzyme activity and kinetics of bacterial growth with an inducer (IPTG) and without inducer were determined. The maximum enzymatic activity was observed at 7 h post induction with a value of 3740 ± 342 U/L, which was similar to that reported for the nat...

Some Basidiomycota were chosen for studies of key ligninases synthesis (25°C, 30 days) in modified medium (shaken or not cultures) with added wheat straw. Liquid Czapek medium with straw yielded a higher amount of laccase than peroxidase, ground straw induced enzyme worse than chopped straw. With peroxidase the reverse dependencies were observed. Laccase of Lentinus edodes synthesized two enzym...