The isolation and identification of labeled amino acids from the acid hydrolysates of cells of Clostridium kluyveri grown in synthetic medium containing ethanol, acetate, and CO* as carbon sources, and with Cl402 or acetate-l-Cl4 as labeled substrate, have been reported previously (28). Alanine, serine, glycine, threonine, and aspartic acid so obtained have been degraded to determine the positi...

was obtained with normal horse serum, which does not have antilymphoma effects and is known to be devoid of asparaginase. By contrast, under identical conditions in vivo, the 6C3HED cells failed to take up L-aspartic acid-14C,or did so only poorly. In vitro, the Lymphoma 6C3HED-OG cells also took up Lasparagine-14C avidly. Again, the incorporation of L-asparagineJ4C was almost completely preven...

was obtained with normal horse serum, which does not have antilymphoma effects and is known to be devoid of asparaginase. By contrast, under identical conditions in vivo, the 6C3HED cells failed to take up L-aspartic acid-14C,or did so only poorly. In vitro, the Lymphoma 6C3HED-OG cells also took up Lasparagine-14C avidly. Again, the incorporation of L-asparagineJ4C was almost completely preven...

Pepsin reacts stoicheiometrically with the active-site-directed irreversible inhibitor N-diazoacetyl-l-phenylalanine methyl ester, with concomitant loss of all proteolytic and peptidolytic activity. The reagent esterifies a unique aspartic acid residue in pepsin, which is in the sequence:Ile-Val-Asp-Thr-Gly-Thr-Ser

Aspartyl and asparaginyl deamidation, isomerization, and racemization reactions have been studied in synthetic peptides to model these spontaneous processes that alter protein structure and function. We show here that the peptide L-Val-L-Tyr-L-Pro-L-Asn-Gly-L-Ala undergoes a rapid deamidation reaction with a half-life of only 1.4 days at 37 degrees C, pH 7.4, to give an aspartyl succinimide pro...

A series of stable acyclic aminals derived from L-(+)-aspartic acid were synthesized in excellent yields (up to 96%) and characterized by spectroscopic methods. They were applied as enantioselective catalysts in Henry reactions of nitromethane with various aldehydes in the presence of Cu(II) ions, affording the corresponding adducts in high yields (up to 90%) and enantioselectivities (up to 92%...

The enzyme activities of four strains of Legionella pneumophilia were investigated by using the API ZYM system (API System S.A., F-38390 Montalieu Vercieu, France) and synthetic substrates. Aminopeptidases were detected specifically against L-alanine, L-arginine, L-aspartic acid, L-cystine, L-glutaminic acid, glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine...