In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...

This paper reports a new simple, sensitive and selective catalytic kinetic spectrophotometric method for the determination of trace quantities of Os(VIII). Janus Green is oxidized by hydrogen peroxide in borate buffer at pH 9.0 and Os(VIII) catalyzes the reaction. Reaction rate was monitored spectrophotometrically at 618 nm. The calibration graph is linear for 0.4 – 430.0 ng mL-1 of Os(VIII) an...

A validate rapid, economical and sensitive visible spectrophotometric method has been developed for quantitative determination of amprenavir in bulk drug and formulation samples. This method is validated for irinotecan with chromogenic reagent 3-methyl benzothiazolinone hydrazine (MBTH). The calibration curve was linear over Beer’s concentration range of 25-350 μg/ml. The relative standard devi...

First-ever three spectrophotometric methods using sulfanilamide (SAA), sulfadoxine (SDX) and sulfamethoxazole (SMX), the widely used sulfa drugs as new class of coupling agents for the spectrophotometric determination of cardol, a phenolic compound found in cashew nut shell liquid, a by-product of cashew industry is proposed. The methods are based on the interaction of diazotized sulfa drugs wi...

Using UV-visible spectrophotometric data of (1:1), electron donor-acceptor complexes (AD1, AD2 and AD3) have been investigated. The complexes concerned are n-butyl amine (D1) and cyclohexyl amine (D2) and phenyl hydrazine (D3) as electron donors with iron (III) ion as an electron acceptor (A) in methanol at 25°C. The values of formation constant (kAD), molar extinction coefficient (eAD), and ab...

Um método cinético catalítico espectrofotométrico, simples, seletivo e sensível para a determinação de quantidade traço de pesticidas organofosforados foi proposto. O método é baseado no efeito catalítico de pesticidas organofosforados (malationa, dimetoato e forato) na oxidação de LCV (leuco cristal violeta) por iodato de potássio em meio de ácido clorídrico, produzindo um corante violeta. O c...

Two simple, rapid and sensitive spectrophotometric methods for the determination of three fluoroquinolones, namely levofloxacin, norfloxacin and ciprofloxacin have been performed either in pure form or in their tablets. In the first method, levofloxacin and norfloxacin are directly treated with bromocresol green (BCG) in dichloromethane while ciprofloxacin is allowed to react with the same dye ...

Seven drugs viz., Paroxetine, Famotidine, Quetiapine, Dextromethorphan, Levofloaxacin, Irbesartan and Itraconazole were tested for the formation of charge transfer complexes with DDQ and the interaction formed a basis for spectrophotometric determination of the drugs. Acetonitrile was found to be suitable solvent for the analysis. The methods have been validated in terms of ICH guidelines The c...

The spectrophotometric and microbiological assay methods used for the determination of ampicillin levels in ampicillin/cloxacillin combinations were compared. A total of 24 samples of ampicillin/cloxacillin suspensions from 8 batches and 3 companies (B2, D and E) were assayed for their ampicillin content levels using the Spectrophotometric assay method. Infra-red (IR) Spectroscopy confirmed the...

Macrolides, one of the most commonly used class of antibiotics, are a group of drugs produced by Streptomyces species. They belong to the polyketide class of natural products. Their activity is due to the presence of a large macrolide lactone ring with deoxy sugar moieties. They are protein synthesis inhibitors and broad-spectrum antibiotics, active against both gram-positive and gram-negative ...