The activity of y-glutamyltransferase (EC 2.3.2.2) is measured on the DuPont aca by an adaptation of the Szasz procedure ;-y-glutamyl-p-nitroanilide is transferred to glycylglycine, thereby releasing p-nitroaniline, which is measured at 405 nm. I have noticed low glutamyltransfer-ase activity in patients with cholestasis wherealkaline phosphate (EC 3.1.3.1) is increased, total bilirubin> 100 mg...

The apparent unbound bilirubin concentration by the “peroxidase” method (U) and the total unconjugated bilirubin in blood (T), albumin-bound bilirubin (B), and reserve bilirubin binding capacity (A) by the bilirubin hematofluorometer were measured in 164 specimens from 98 neonates and in a series of artifactual specimens, made by adding bilirubin to the blood of a single adult donor. Linear cor...

OBJECTIVE The recognition, follow-up, and early treatment of neonatal jaundice has become more difficult, since the earlier discharge of newborns from hospitals has become common practice. This prospective study was undertaken to identify the newborns at risk for developing significant hyperbilirubinemia later during the first days of life by measuring the serum bilirubin levels of the first 5 ...

1. A multicompartmental model of erythrokinetics and bilirubin production has been developed to predict the consequences of chronic phlebotomy on daily bilirubin turnover. 2. Control values for four physiological variables including bilirubin turnover were determined in a 20-year-old woman with type I congenital nonhaemolytic jaundice (Crigler-Najjar syndrome). With these base-line data, the mo...

The results of spectral-fluorescent and diffuse-optical studies living tissues under the effect low-temperature atmospheric pressure plasma jet in a mixture air argon are presented. Potential fluorophores identified coefficients optical absorption transport scattering determined. It was revealed irradiation tissue leads to an increase intensity fluorescence diffuse reflection coefficient, which...

Sodium phenobarbital and various hormones, compounds capable of hepatic enzyme induction, were given to an infant boy with congenital, nonhemolytic, unconjugated, hyperbilirubinemia and severe kernicterus for prolonged periods between the ages of 2 and 25 months to determine their effect on serum bilirubin concentrations. Phenobarbital, 5 mg/day orally, on two occasions decreased serum bilirubi...