This study investigates the effect of steroid and polypeptide hormones on human NK cell activity in vitro. Solutions of hormones were incubated with normal peripheral blood cells in a 4 h cytotoxic assay against the NK-sensitive erythromyeloid cell line K562. The steroid hormones oestradiol, progesterone and testosterone did not exhibit a significant effect on K562 lysis at concentrations withi...

The unexpected enhancement of alkalinee phosphatase (ALP) activity in the serum of a group of STZ-diabetic rats recieving the Teucrium polium extract (with hypoglycaemic property) may be attributed to its probable side effects. In order to get a more precise knowledge about this subject, we evaluated the effect of T. polium extract on the plasma membrane ALP activity of K562 cell line. Our data...

Nilotinib, a second-generation tyrosine kinase inhibitor (TKI), has been approved for first-line chronic myeloid leukemia (CML) treatment. The improved clinical response of nilotinib over that of the first generation TKI, imatinib, has been thought to be a result of its high potency of inhibition of BCR-ABL kinase. This study aimed to characterize differences between nilotinib and imatinib in t...

Introduction: Chronic myelogenous leukemia (CML) is a myeloproliferative disorder caused by the Philadelphia chromosome translocation, at (9; 22), which results in BCR-ABL fusion tyrosine kinase oncoprotein. This fusion induces down-regulation of miR-155. Upregulation of miR-155 can influence cell fate via the effect on p27kip1 and apoptosis. The aim of this study was to induce apoptosis in K56...

We have compared the expression of the ras protooncogene family (H-, K-, and N-ras) in leukemia cell differentiation utilizing as a model K562 and HEL erythroleukemia cells treated either with 1-beta-arabinofuranosylcytosine or 12-O-tetradecanoylphorbol-13-acetate (TPA). 1-beta-D-Arabinofuranosylcytosine induced terminal erythroid differentiation of K562 cells, while TPA induced myeloid differe...

Objective: The effects of microRNA-20a (miR-20a) antisense oligonucleotides (ASODNs) on the proliferation and apoptosis of K562 cells were investigated, and the effects of these ASODNs in combination with imatinib on K562 cells were preliminarily observed. Methods: miR-20a ASODNs and scrambled oligonucleotides (SODNs) were chemically synthesized, and the later was used as the control. miR-20a A...