following the categorical approach to universal algebra through algebraic theories, proposed by f.~w.~lawvere in his phd thesis, this paper aims at introducing a similar setting for general topology. the cornerstone of the new framework is the notion of emph{categorically-algebraic} (emph{catalg}) emph{topological theory}, whose models induce a category of topological structures. we introduce t...

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

in his perspective “navigating between stealth advocacy and unconscious dogmatism: the challenge of researching the norms, politics and power of global health,” ooms argues that actions taken in the field of global health are dependent not only on available resources, but on the normative premise that guides how these resources are spent. this comment sets out how the application of a predomina...

Nuclear forces have been studied in depth over the past fifty years, leading to excellent phenomenological descriptions. A recent resurgence of interest in this field has been fueled by the promise of systematic results from an effective field theory (EFT) analysis [1]. EFT techniques adapted to many-body systems may give new insight into the properties of nuclei and their connection to QCD. Th...

In this paper are presented notes on the primary types of some species oil-collecting bees genus Centris described by European naturalists and entomologists Amédée Louis Michel Lepeletier de Saint-Fargeau, Anders Christian Jensen-Haarup, Arthur Marie Joseph Vachal, Charles Émile Blanchard, Embrik Strand, Félix Édouard Guérin-Méneville, Guillaume-Antoine Olivier, Jean Guillaume Audinet-Serville,...

Unconscious mental processes have recently started gaining attention in a number of scientific disciplines. One of the theoretical frameworks for describing unconscious processes was introduced by Jung as a part of his model of the psyche. This framework uses the concept of archetypes that represent prototypical experiences associated with objects, people, and situations. Although the validity ...