Carbonic anhydrase (CA) is a multi-functional enzyme that catalyzes the hydration/dehydration of carbon dioxide. In the red blood cell (rbc), CA is necessary to facilitate the transport of carbon dioxide out of the body. Results from earlier biochemical studies indicate that ancient vertebrates, such as agnathans, possess a low activity rbc CA isozyme, whereas more recently evolved vertebrates,...

Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic ...

Vulnerabilities in biometric systems including spoofing has emerged as an important issue. The focus of this work is on characterization of ‘perspiration pattern’ in a time-series of fingerprint images for liveness detection. By using information in the high pass bands of the images the similarity score for the two images is calculated to determine the uniqueness of the perspiration pattern. In...

The diversity of the predominant bacteria in the human gastrointestinal tract was studied by using 16S rRNA-based approaches. PCR amplicons of the V6 to V8 regions of fecal 16S rRNA and ribosomal DNA (rDNA) were analyzed by temperature gradient gel electrophoresis (TGGE). TGGE of fecal 16S rDNA amplicons from 16 individuals showed different profiles, with some bands in common. Fecal samples fro...

S-Adenosylmethionmne synthetase isozymes (EC 2.5.1.6) were studied in hepaticnodules,primaryhepatocellularcarci nomas, and various transplantable hepatocellulan carcinomas induced by acetylaminofluomene. With the exception of hepa toma 7800, the intermediate-Km isozyme was the only enzyme species detectable in transplantable hepatomas, including 7777, 31 i C, 253, 252, 56, and Novikoff hepatoma...

Objective: To improve the strain of Pleurotus mushroom by protoplast fusion technique Methods: The white rot fungus Pleurotus species was isolated from fresh fruiting body of Oyster mushroom by spore print technique and was cultured on Potato dextrose agar. The selected strain was identified based on the morphological and cultural characters on Potato dextrose agar. The protoplasts were isolate...

Powdery mildew infection in cucumber plants was significantly reduced by foliar application of 2,6dichloroisonicotinic acid (INA). Although the overall isozyme banding patterns of superoxide dismutase (SOD) and peroxidase (POD) were very similar, there were differences in the densities of major bands between the leaves of control and INA-applied plants. When plants were subjected to foliar appl...

Glutamate receptors and calcium have been implicated as triggering factors in the induction of tolerance by ischemic preconditioning (IPC) in the brain. However, little is known about the signal transduction pathway that ensues after the IPC induction pathway. The main goals of the present study were to determine whether NMDA induces preconditioning via a calcium pathway and promotes translocat...

The histamine H2-receptor antagonists have been identified as inhibitors of human liver aldehyde dehydrogenase (EC 1.2.1.3) isozymes, E1, E2, and E3. Inhibition was strongest with the E3 isozyme, whose substrates include gamma-aminobutyraldehyde, the aldehyde metabolites of polyamines, and betaine aldehyde. Burimamide, metiamide, cimetidine guanidine, cimetidine, and tiotidine were competitive ...