A screening test was undertaken to isolate microorganisms that produced ascorbate exidase. The E".Z,IMqe.a.C`llg'.W.ai,l,:,dit:iaEh"S".2e,,fi,l・g"ti",`&Of,?・,fuig,a,i,2tr;IL",(:・ld:-,l5,2u,"eIIYIS,O,i,a.`e.d.I・i.O.M,a,FO"i,a.MP,Lea examinations of cultural conditions, optintum conditions for enzyme production were found; strain HI-25 was aerobically cultured by a jar fermenter at 250C in a rned...

Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...

In cell-separation technology the term ‘component’ of a mixture corresponds to a group of cells, which is usually called a cell population and shares a number of common features. How many common features a group of cells has to share in order to be called a cell population depends on the interest of the ‘separator’. For example, a T-cell population may be a group of mononuclearwhite blood cells...

Rapid, efficient, and economic method for the isolation and purification of islets has been pursued by numerous islet-related researchers. In this study, we compared the advantages and disadvantages of our developed patented method with those of commonly used conventional methods (Ficoll-400, 1077, and handpicking methods). Cell viability was assayed using Trypan blue, cell purity and yield wer...

The purpose of this study was to isolate and characterize Proline Dehydrogenase (ProDH) enzyme frommicroorganisms isolated from soil in Iran. Isolation and screening of L-proline degradative enzymes from soilsamples was carried out. The isolate was characterized by biochemical markers and 16S rRNA geneanalysis. The target ProDH was purified and the effects of pH and temperatur...

Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would enable the one donor-one recipient concept and improve the therapeutic effects of islet transplantation. This study aims to improve islet transplantation by increasing the yield of islets fr...