A 3-year-old pregnant Arabian mare was referred to the Veterinary Teaching Hospital of Shahid Chamran University of Ahvaz with a history of bleeding and rodenticide ingestion. The results of paraclinical examinations showed severe normocytic and normochromic anemia, decreased serum total protein, albumin, and fibrinogen concentrations, increased serum total bilirubin, urea, and creatinine conce...

Doxorubicin (DXR) is a widely used and efficient anticancer drug. However, its application is limited by the risk of severe cardiotoxicity. Impairment of cardiac high-energy phosphate homeostasis is an important manifestation of both acute and chronic DXR cardiotoxic action. Using the Langendorff model of the perfused rat heart, we characterized the acute effects of 1-h perfusion with 2 or 20 m...

The study was aimed at assessing the total enzyme activity and the profile of breast cancer and dysplasia on the human material. In addition, the validity of data was evaluated from the aspect of improving diagnostics. Lactate dehydrogenase activity, as well as the profile of its isoenzymes, pyruvate kinase and hexokinase, were measured. The study included 60 samples of breast cancer, out of wh...

Three surveys of the measurement and interpretation of creatine kinase (CK; EC 2.7.3.2) isoenzyme 2 (CK-MB) were conducted in Ontario, Canada, in 1989. Of the clinical laboratories participating, 66% used immunological methods and 24% used electrophoretic methods. Although reference ranges and interpretative routines varied widely, 95% of the laboratories reported correct interpretations for 10...

We present a case where the laboratory was asked to investigate suspected interference in our troponin T assay. The patient 29-year-old male with schizophrenia on long term clozapine. Hs-Troponin (Roche), performed routine monitoring chronically elevated between 40 100 ng/L (RI <14 ng/L), multiple cardiac investigations being normal. Creatine kinase similarly 400 700 U/L <250 U/L) over same tim...

acetone fractionated peroxidase from crude extract of brassica oleracea leaves (cabbage) was purified in three steps on chromatographic columns, using sp-sepharose, deae-sepharose and con a-sepharose. the specific activity of purified main isoenzyme (boc-pod) is 1887 u/mg protein with rz: 3.1, which is 172 times more than the rz of crude extract with 4.3% recovery. the molecular weight of boc-p...

(b) by adding antiserum to the B sub-unit of CK to a portion of a patient's sample and the control, then electro-phoresing the untreated and treated aliquots in adjacent positions on the same agarose film. On inspecting the films before enzyme substrate was added, we would see fluorescent bands cathodal and anodal, adjacent to but distinct from the position where the BB isoenzyme would be expec...

Twenty five blood samples from 6-month to 5-year old children with visceral leishmaniasis were analysed for various enzymes. Aspartate transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LD) and its various isoenzymes were estimated in the serum, while glucose 6-phosphate dehydrogenase (G6PD) was measured in red blood cells. For comparison, blood samples from healthy chi...