This report describes two patients who presented acute disseminated and severe toxoplasmosis as the first opportunistic disease related to acquired immunodeficiency syndrome. At admission, clinical and laboratory findings were similar to sepsis or septic shock and a fast evolutive course to death occurred in both cases. At necropsy, an inflammatory reaction and presence of a great number of Tox...

Four Korean squirrels (Tanias siberius) imported in Spain from People's Republic of China died 2 days after their arrival at a pet shop. They had neurological signs associated with generalized toxoplasmosis involving brain, lungs, liver, and the heart. Toxoplasma gondii-like tachyzoites and tissue cysts were found in organs of all four squirrels. The protozoa stained positively with T. gondii p...

We studied the sensitivity and specificity of PCR to detect T. gondii DNA by aliquoting various concentration of tachyzoites into laboratory specimens from 60 positive and 10 negative buffy-coat samples. We were able to detect the specific gene from purified DNA samples containing as few as 0.25 parasites per 100,000 human leukocytes. These results had an impressive initial 100% specificity but...

a cytogenetic study was performed on m4 generation of gamma irradiated lines of shirpan cultivar of tetraploid cotton (gossypium hirsutum l.). a significant difference was observed in cytogenetical characteristics such as the number and type of quadrivalents as well as percentage of cells showing abnormality among the irradiated and non irradiated lines. cytomixis and post- pachytene diffuse st...

4-methylimidazole (4-mei) is a simple nitrogen-containing heterocyclic compound, and recently classified as group 2b by international agency for research on cancer (iarc). caramel coloring is widely applied food coloring in many food products including coffee, carbonated beverages, beer and wine. in present study, ɣ-irradiation was applied to caramel color iii to reduce the level of 4-mei witho...

Background: Radio-adaptive response and bystander effects are known phenomena occurring in cells following exposure to ionizing radiation (IR). In this study we examined possible radio-adaptation of lymphocytes following bystander effects induced by CHO-K1 cells. Materials and Methods: Whole blood and CHO-K1 cells were cultured in RPMI-1640 complete medium. Cells were separately irradiated with...

Background: Radiation-induced bystander effect is a response which results in damage in non-irradiated cells in response to signals from the irradiated cells. The aim of the present study was to investigate microwave-induced bystander effect from a GSM mobile phone simulator on induction of apoptosis in Jurkat cell line. Materials and Methods: Jurkat cells were divided into three groups of non-...

background: diagnosis of some diseases is difficult due to invasive sampling. urine has been candi-date as a non-invasive and convenient alternative. it has many advantages and easy accessibility but some technical ills should be removed. finding a suitable extraction method for improving urine dna quantity and quality in altering invasive specimens for molecular diagnosis of some infectious di...

background: we evaluated the in vivo activity of bunium persicum (boiss) essential oil on infected mice with acute toxoplasmosis. methods: to evaluate prophylactic effects, male nmri mice received b. persicum essential oil at the concentrations of 0.05 and 0.1 ml/kg for 14 days . after 24 h mice were infected intraperitonealy with 1×10 4 tachyzoites of t. gondii, rh strain. in order to investig...